再生大鼠肝脏中的葡萄糖胺代谢
Glucosamine metabolism in regenerating rat liver.
作者信息
Akamatsu N, Nakajima H, Miyata S
出版信息
Biochem J. 1976 Sep 15;158(3):589-92. doi: 10.1042/bj1580589.
Abstract
- Glycoprotein synthesis was investigated with [1-14C]glucosamine in vivo. [14C]Glucosamine was administered intravenously 24h after hepatectomy to rats. 2. Incorporation into the acid-soluble fraction was maximum at 15 min after injection both in sham-operated and hepatectomized rats. 3. Enhancement of incorporation into UDP-N-acetylhexosamine in regenerating liver was observed. However, its specific activity was lower, because of a greater enhancement of synthesis de novo of the amino sugar. 4. In the liver acid-insoluble fraction, maximum incorporation of [14C]glucosamine was at 30 min in sham-operated rats and 2 h in hepatectomized rats respectively. 5. In sham-operated rats, incorporation into the plasma acid-insoluble fraction followed that of the liver acid-insoluble fraction, but hepatectomy resulted in a rapid enchancement of incorporation into plasma. 6. It is concluded that synthesis of liver glycoproteins is stimulated after partial hepatectomy and that glycoproteins synthesized are released rapidly into the plasma.
摘要
- 采用[1-14C]葡糖胺在体内研究糖蛋白的合成。肝切除术后24小时，给大鼠静脉注射[14C]葡糖胺。2. 在假手术组和肝切除组大鼠中，注射后15分钟时，进入酸溶性部分的掺入量均达到最大值。3. 观察到再生肝中进入UDP-N-乙酰己糖胺的掺入量增加。然而，其比活性较低，因为氨基糖的从头合成增加得更多。4. 在肝脏酸不溶性部分，假手术组大鼠中[14C]葡糖胺的最大掺入时间为30分钟，肝切除组大鼠为2小时。5. 在假手术组大鼠中，进入血浆酸不溶性部分的掺入情况与肝脏酸不溶性部分的情况一致，但肝切除术导致血浆中的掺入迅速增加。6. 得出结论，部分肝切除术后肝脏糖蛋白的合成受到刺激，且合成的糖蛋白迅速释放到血浆中。
相似文献
1
Glucosamine metabolism in regenerating rat liver.再生大鼠肝脏中的葡萄糖胺代谢
Biochem J. 1976 Sep 15;158(3):589-92. doi: 10.1042/bj1580589.
2
Synthesis in vitro of glycoprotein in regenerating rat liver.再生大鼠肝脏中糖蛋白的体外合成
Biochim Biophys Acta. 1977 Jul 21;498(1):272-81. doi: 10.1016/0304-4165(77)90265-3.
3
Synthesis in vitro of glycosaminoglycans in regenerating rat liver.再生大鼠肝脏中糖胺聚糖的体外合成
J Clin Chem Clin Biochem. 1981 Jul;19(7):465-9. doi: 10.1515/cclm.1981.19.7.465.
4
Metabolism of sialic acid in regenerating rat liver.再生大鼠肝脏中唾液酸的代谢
Biochem J. 1980 Jun 15;188(3):905-11. doi: 10.1042/bj1880905.
5
Serum glycoprotein synthesis after partial hepatectomy in the rat.大鼠部分肝切除术后血清糖蛋白的合成
Biochem J. 1976 Jul 15;158(1):153-5. doi: 10.1042/bj1580153.
6
Galactose metabolism in regenerating rat liver.再生大鼠肝脏中的半乳糖代谢
Biochem J. 1976 Jan 15;154(1):141-7. doi: 10.1042/bj1540141.
7
Insulin-like growth factor-II/mannose-6-phosphate receptors are increased in hepatocytes from regenerating rat liver.胰岛素样生长因子-II/甘露糖-6-磷酸受体在再生大鼠肝脏的肝细胞中增加。
Endocrinology. 1990 May;126(5):2543-9. doi: 10.1210/endo-126-5-2543.
8
Partially hepatectomized rats: a model for the study of the effect of toxins on the plasma protein profiles of nascent hepatocytes.部分肝切除大鼠：用于研究毒素对新生肝细胞血浆蛋白谱影响的模型。
J Toxicol Environ Health. 1992 May;36(1):43-57. doi: 10.1080/15287399209531622.
9
The effect of increasing nucleotide-sugar concentrations on the incorporation of sugars into glycoconjugates in rat hepatocytes.增加核苷酸糖浓度对大鼠肝细胞中糖掺入糖缀合物的影响。
Biochem J. 1995 Feb 1;305 ( Pt 3)(Pt 3):865-70. doi: 10.1042/bj3050865.
10
[Effects of 3,4,5-trimethoxy-N-(3-piperidyl) benzamide (KU-54) on the incorporation (excretion) of 14C-glucosamine in the gastric mucosa and the liver of rats].
Nihon Yakurigaku Zasshi. 1984 Jul;84(1):11-8.
引用本文的文献
1
Novel biochemical pathways for 5-Fluorouracil in managing experimental hepatocellular carcinoma in rats.5-氟尿嘧啶管理实验性大鼠肝癌的新生化途径。
J Membr Biol. 2010 Mar;234(1):29-34. doi: 10.1007/s00232-010-9236-7. Epub 2010 Feb 23.
2
Accumulation of [3H]sialyl-conjugates in sialidosis (sialidase-deficient) fibroblasts cultured in the presence of [3H]-N-acetylmannosamine.在存在[³H]-N-乙酰甘露糖胺的情况下培养的涎酸沉积症（唾液酸酶缺乏）成纤维细胞中[³H]唾液酸共轭物的积累。
J Inherit Metab Dis. 1986;9(1):79-88. doi: 10.1007/BF01813907.
3
The UDP-N-acetylglucosamine-asparagine-sequon N-acetyl-beta-D-glucosaminyltransferase activity in preparations of rough endoplasmic reticulum from regenerating rat liver.来自再生大鼠肝脏的糙面内质网制剂中的UDP-N-乙酰葡糖胺-天冬酰胺序列N-乙酰-β-D-葡糖胺基转移酶活性
Biochem J. 1977 Apr 15;164(1):257-61. doi: 10.1042/bj1640257.
4
Utilization by the isolated perfused rat liver of N-acetyl-D-[1-14C]galactosamine and N-[3H]acetyl-D-galactosamine for the biosynthesis of glycoproteins.离体灌注大鼠肝脏对 N-乙酰-D-[1-¹⁴C]半乳糖胺和 N-[³H]乙酰-D-半乳糖胺用于糖蛋白生物合成的利用情况。
Biochem J. 1978 Aug 15;174(2):421-6. doi: 10.1042/bj1740421.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
3
4
Synthesis of plasma albumin and tissue proteins in regenerating liver.再生肝脏中血浆白蛋白和组织蛋白的合成。
Metabolism. 1962 Sep;11:957-66.
5
A modified colorimetric method for the estimation of N-acetylamino sugars.一种用于估算N-乙酰氨基糖的改良比色法。
J Biol Chem. 1955 Dec;217(2):959-66.
6
Hepatocellular ultrastructure during liver regeneration after subtotal hepatectomy.肝大部切除术后肝脏再生过程中的肝细胞超微结构
Exp Mol Pathol. 1966 Oct;5(5):455-74. doi: 10.1016/0014-4800(66)90026-8.
7
Plasma protein biosynthesis following laparotomy and partial hepatectomy.
Proc Soc Exp Biol Med. 1970 Nov;135(2):415-8. doi: 10.3181/00379727-135-35064.
8
Synthesis of intestinal glycoprotein. Incorporation of (I- 14 C) glucosamine in vitro.肠糖蛋白的合成。体外（I-14C）葡糖胺的掺入。
Biochim Biophys Acta. 1971 Feb 28;261(2):353-64.
9
Formation of glucosamine 6-phosphate in regenerating rat liver.
Biochim Biophys Acta. 1971 Aug 19;244(2):311-7. doi: 10.1016/0304-4165(71)90231-5.
10
Studies on hog spleen N-acetylglucosamine kinase. I. Purification and properties of N-acetylglucosamine kinase.猪脾N-乙酰葡糖胺激酶的研究。I. N-乙酰葡糖胺激酶的纯化及性质
Biochim Biophys Acta. 1970 Oct 14;220(1):51-60. doi: 10.1016/0005-2744(70)90228-7.
Zotero 插件