Maurer D R, Sielczak M, Oliver W, Abraham W M, Wanner A
J Appl Physiol Respir Environ Exerc Physiol. 1982 Apr;52(4):1018-23. doi: 10.1152/jappl.1982.52.4.1018.
The purpose of this investigation was to determine if abnormal ciliary function contributes to allergic mucociliary dysfunction. In conscious sheep with Ascaris suum hypersensitivity, ciliated cells were obtained with a cytology brush and tracheal mucous velocity (TMV) was determined before and serially for 2 h following antigen inhalation. The recovered cells (also containing mast cells) were suspended in a chamber, and ciliary activity was viewed microscopically and recorded on videotape for subsequent slow-motion analysis of ciliary beat frequency (CBF). One hour after A. suum challenge mean CBF (+/- SE) showed a slight increase from a base-line value of 630 +/- 16 to 716 +/- 30 beats/min (P less than 0.05) when mean TMV was decreased to 57% of base line (P less than 0.05). After 2 h, both mean CBF and TMV returned toward base line. Since possible in vivo actions of chemical mediators liberated by antigen challenge may have been lost after suspension of the brushed cells, we also assessed the effects of antigen on CBF in vitro. A. suum caused a dose-dependent increase in CBF that was blocked by cromolyn sodium. We conclude that 1) allergic mucociliary dysfunction is not caused by a decrease in CBF and 2) antigen-induced release of chemical mediators increases CBF.
本研究的目的是确定异常的纤毛功能是否会导致过敏性黏液纤毛功能障碍。在对猪蛔虫过敏的清醒绵羊中，用细胞学刷获取纤毛细胞，并在吸入抗原前及之后连续2小时测定气管黏液速度（TMV）。回收的细胞（也含有肥大细胞）悬浮在一个小室中，通过显微镜观察纤毛活动，并录制在录像带上，以便随后对纤毛摆动频率（CBF）进行慢动作分析。猪蛔虫攻击1小时后，平均CBF（±标准误）从基线值630±16轻微增加到716±30次/分钟（P<0.05），而平均TMV降至基线的57%（P<0.05）。2小时后，平均CBF和TMV均恢复至基线水平。由于抗原攻击释放的化学介质在刷取的细胞悬浮后可能已失去其可能的体内作用，我们还在体外评估了抗原对CBF的影响。猪蛔虫导致CBF呈剂量依赖性增加，这一增加被色甘酸钠阻断。我们得出结论：1）过敏性黏液纤毛功能障碍不是由CBF降低引起的；2）抗原诱导的化学介质释放会增加CBF。
