根瘤菌菜豆根瘤菌结瘤缺陷型的正向选择
Positive selection of nodulation-deficient Rhizobium phaseoli.
作者信息
Raleigh E A, Signer E R
出版信息
J Bacteriol. 1982 Jul;151(1):83-8. doi: 10.1128/jb.151.1.83-88.1982.
Abstract
A high proportion of Rhizobium phaseoli mutants that survived infection with phage F1 were found to be nodulation deficient. Two that were examined in detail had internal defects in addition to the expected surface defects. One internal defect was in the enzyme phosphoglucose isomerase. The use of phages to select appropriate mutants should apply generally in any system in which surface components are involved.
摘要
在感染噬菌体F1后存活下来的菜豆根瘤菌突变体中，发现有很大比例的突变体结瘤能力不足。详细研究的两个突变体除了预期的表面缺陷外，还存在内部缺陷。其中一个内部缺陷存在于磷酸葡萄糖异构酶中。利用噬菌体来筛选合适的突变体，在任何涉及表面成分的系统中都应普遍适用。
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