Johansen E, Finan T M, Gefter M L, Signer E R
J Bacteriol. 1984 Oct;160(1):454-7. doi: 10.1128/jb.160.1.454-457.1984.
Monoclonal antibodies were produced to the surface of the symbiotic nitrogen-fixing bacterium Rhizobium meliloti. Bacterial lysis in the presence of complement or cycles of agglutination and growth were used to select mutants no longer recognized by the antibodies. The mutants were used to produce new antibodies with different specificities. Several mutants had altered sensitivity to one or more bacteriophages. R. meliloti strains from different sources had distinct patterns of sensitivity to monoclonal antibodies and phages, which together can be used for discriminative typing.
制备了针对共生固氮细菌苜蓿根瘤菌表面的单克隆抗体。利用补体存在下的细菌裂解或凝集与生长循环来筛选不再被抗体识别的突变体。这些突变体被用于产生具有不同特异性的新抗体。几个突变体对一种或多种噬菌体的敏感性发生了改变。来自不同来源的苜蓿根瘤菌菌株对单克隆抗体和噬菌体具有不同的敏感性模式，两者结合可用于鉴别分型。
