Seitz T L, Noonan K D, Hench L L, Noonan N E
J Biomed Mater Res. 1982 May;16(3):195-207. doi: 10.1002/jbm.820160303.
We have demonstrated that an established hamster cell line (NIL 8 M-2) will adhere to the bioceramic bioglass. The rate at which the NIL 8 M-2 cells assume a spread morphology on bioglass is density dependent and the morphology displayed by NIL 8 M-2 cells attached to bioglass is much more elongated than that displayed by NIL 8 M-2 cells attached to nonreactive glass. Precoating the bioglass with the plasma form of human fibronectin significantly reduces the density dependent nature of cell spreading. Coating the bioglass with fibronectin also reduces the time required for cell spreading and changes the morphology of the attached cells from an elongated to an extremely flattened shape. Our work raises the possibility that bone-implant adhesion might be improved by introducing molecules relevant to cell-substrate attachment into the biomaterial prior to implantation.
我们已经证明，一种已建立的仓鼠细胞系（NIL 8 M-2）会黏附于生物陶瓷生物玻璃上。NIL 8 M-2细胞在生物玻璃上呈现铺展形态的速率取决于细胞密度，并且附着在生物玻璃上的NIL 8 M-2细胞所呈现的形态比附着在非活性玻璃上的NIL 8 M-2细胞所呈现的形态要长得多。用人纤连蛋白的血浆形式预包被生物玻璃可显著降低细胞铺展的密度依赖性。用纤连蛋白包被生物玻璃还可减少细胞铺展所需的时间，并使附着细胞的形态从细长形变为极扁平形。我们的工作提出了一种可能性，即在植入前将与细胞-基质附着相关的分子引入生物材料中，可能会改善骨-植入物的黏附。
