Krstulović A M
J Chromatogr. 1982 Apr 16;229(1):1-34. doi: 10.1016/s0378-4347(00)86033-8.
High-performance liquid chromatography, particularly in its reversed-phase mode, coupled with electrochemical or fluorometric detection, is becoming increasingly popular as an analytical tool for metabolic profiling of substances of neurochemical interest, such as catecholamines and their metabolites. During the last decade, a continued effort has been made to improve and simplify the analytical methodology for routine use in clinical laboratories where this technique is tremendously needed. New developments in column technology, reliable detectors, simplified sample cleanup procedures, and particularly better understanding of the complex physicochemical phenomena underlying the operation of electrochemical detection, have resulted in a steady and encouraging progress. The purpose of this review was to describe the current analytical methodology and recent applications of HPLC in the field of catecholamine metabolism. Although this discussion is by no means detailed and complete, it, at least, hints at the impact of this technique on biochemical investigations and its future potential in clinical laboratories.
高效液相色谱法，尤其是反相模式，结合电化学或荧光检测，作为一种分析工具，在对神经化学相关物质（如儿茶酚胺及其代谢物）进行代谢谱分析方面正变得越来越受欢迎。在过去十年中，人们持续努力改进和简化该分析方法，以便在急需此技术的临床实验室中常规使用。柱技术的新发展、可靠的检测器、简化的样品净化程序，尤其是对电化学检测操作背后复杂物理化学现象的更好理解，都带来了稳步且令人鼓舞的进展。这篇综述的目的是描述高效液相色谱法在儿茶酚胺代谢领域的当前分析方法和近期应用。尽管本讨论绝非详尽和完整，但它至少暗示了该技术对生化研究的影响及其在临床实验室中的未来潜力。
