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肌间神经元在组织培养中表现出电生理和形态学上的多样性。

Myenteric neurons express electrophysiological and morphological diversity in tissue culture.

作者信息

Hanani M, Baluk P, Burnstock G

出版信息

J Auton Nerv Syst. 1982 Mar;5(2):155-64. doi: 10.1016/0165-1838(82)90036-4.

DOI:10.1016/0165-1838(82)90036-4
PMID:7086067
Abstract

Intracellular recordings were taken from neurons of the myenteric plexus of the guinea-pig caecum grown in tissue culture. Subsequently horseradish peroxidase (HRP) was injected into the same neurons for examination by light and electron microscopy. Neurons showed a variety of electrophysiological responses, including S- and AH-cell type behavior, similar to those reported for myenteric neurons in situ, and depolarization to iontophoresis of acetylcholine. Neurons resembling Dogiel type I, type II and intermediate forms, and nerve varicosities which contained small agranular, large granular or large opaque vesicles were observed.

摘要

对在组织培养中生长的豚鼠盲肠肌间神经丛的神经元进行细胞内记录。随后,将辣根过氧化物酶(HRP)注入同一神经元,以便通过光学显微镜和电子显微镜进行检查。神经元表现出多种电生理反应,包括S型和AH型细胞行为,类似于原位肌间神经元的报道,以及对乙酰胆碱离子电渗的去极化反应。观察到类似第I型、第II型和中间型多极神经元的神经元,以及含有小清亮型、大颗粒型或大致密核心型囊泡的神经膨体。

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