Song Z M, Brookes S J, Llewellyn-Smith I J, Costa M
Department of Physiology, Flinders University of South Australia, Adelaide.
Cell Tissue Res. 1995 Jun;280(3):627-37. doi: 10.1007/BF00318365.
External muscle and myenteric plexus from the small intestine of adult guinea-pigs were maintained in vitro for 3 or 6 days. Myenteric neurons and smooth muscle cells from such organotypic cultures were examined at the electron-microscopic level. An intact basal lamina was found around the myenteric ganglia and internodal strands. Neuronal membranes, nuclei and subcellular organelles appeared to be well preserved in cultured tissues and ribosomes were abundant. Dogiel type-II neurons were distinguishable by their elongated electron-dense mitochondria, numerous lysosomes and high densities of ribosomes. Vesiculated nerve profiles contained combinations of differently shaped vesicles. Synaptic membrane specializations were found between vesiculated nerve profiles and nerve processes and cell bodies. The majority of nerve fibres were well preserved in the myenteric ganglia, in internodal strands and in bundles running between circular muscle cells. No detectable changes were found in the ultrastructure of the somata and processes of glial cells. Longitudinal and circular muscle cells from cultured tissue had clearly defined membranes with some close associations with neighbouring muscle cells. Caveolae occurred in rows that ran parallel to the long axis of the muscle cells. These results indicate that the ultrastructural features of enteric neurons and smooth muscle of the guinea-pig small intestine are well preserved in organotypic culture.
将成年豚鼠小肠的外肌层和肌间神经丛进行体外培养3或6天。对来自此类器官型培养物的肌间神经元和平滑肌细胞进行电子显微镜水平的检查。在肌间神经节和节间束周围发现了完整的基膜。培养组织中的神经元膜、细胞核和亚细胞器似乎保存良好，核糖体丰富。多极II型神经元可通过其细长的电子致密线粒体、大量溶酶体和高密度核糖体来区分。含泡状神经形态包含不同形状囊泡的组合。在含泡状神经形态与神经突起和细胞体之间发现了突触膜特化结构。大多数神经纤维在肌间神经节、节间束以及环形肌细胞之间的束中保存良好。在神经胶质细胞的胞体和突起的超微结构中未发现可检测到的变化。培养组织中的纵行肌细胞和环形肌细胞膜清晰，与相邻肌细胞有一些紧密联系。小窝呈平行于肌细胞长轴的行状排列。这些结果表明，豚鼠小肠肠神经元和平滑肌的超微结构特征在器官型培养中保存良好。
