Morison W L, McAuliffe D J, Parrish J A, Bloch K J
J Invest Dermatol. 1982 Jun;78(6):460-3. doi: 10.1111/1523-1747.ep12510144.
The photosensitizing potential of chemicals known to produce photosensitivity in humans was compared to chemicals not considered to be photosensitizers in an in vitro assay. The assay involved exposure of human lymphoid cells to UVA (320-400 nm), and in some cases UVB (280-320 nm) radiation, in the presence of the chemicals and the assessement of phototoxicity as measured by the incorporation of 3[H]-thymidine into nuclear DNA. All known photosensitizers tested were found to be phototoxic, while the nonphotosensitizing agents, with the exception of retinoic acid, were not phototoxic. Peripheral blood mononuclear cells were compared to a T lymphoblastoid cell line as target cells; the latter were superior in terms of convenience, cost and reproducibility of results. This test system has potential as a predictive assay for detecting additional phototoxic chemicals.
在一项体外试验中，将已知会在人体中产生光过敏反应的化学物质的光敏潜力，与那些不被视为光敏剂的化学物质进行了比较。该试验包括在化学物质存在的情况下，将人类淋巴细胞暴露于UVA（320 - 400纳米），在某些情况下还包括UVB（280 - 320纳米）辐射，并通过测量3[H]-胸腺嘧啶核苷掺入核DNA来评估光毒性。所有测试的已知光敏剂都被发现具有光毒性，而除视黄酸外的非光敏剂则没有光毒性。将外周血单核细胞与一种T淋巴母细胞系作为靶细胞进行了比较；就结果的便利性、成本和可重复性而言，后者更具优势。这个测试系统有潜力作为一种预测性试验，用于检测其他光毒性化学物质。
