Mechanism of pigeon liver malic enzyme. Reactivity of class II sulfhydryl groups as a conformational probe for the "half-of-the-sites" reactivity of the enzyme with bromopyruvate.

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作者信息

Pry T A, Hsu R Y

出版信息

Biochemistry. 1978 Sep 19;17(19):4024-9. doi: 10.1021/bi00612a023.

DOI:10.1021/bi00612a023

PMID:708693

Abstract

摘要

相似文献

Mechanism of pigeon liver malic enzyme. Reactivity of class II sulfhydryl groups as a conformational probe for the "half-of-the-sites" reactivity of the enzyme with bromopyruvate.

Biochemistry. 1978 Sep 19;17(19):4024-9. doi: 10.1021/bi00612a023.

Mechanism of pigeon liver malic enzyme: kinetics, specificity, and half-site stoichiometry of the alkylation of a cysteinyl residue by the substrate-inhibitor bromopyruvate.鸽肝苹果酸酶的作用机制：底物抑制剂溴丙酮酸对半胱氨酸残基烷基化的动力学、特异性及半位点化学计量关系

Biochemistry. 1977 Jan 25;16(2):311-20. doi: 10.1021/bi00621a024.

Mechanism of pigeon liver malic enzyme. Modification of sulfhydryl groups by 5,5'-dithiobis(2-nitrobenzoic acid) and N-ethylmaleimide.鸽肝苹果酸酶的作用机制。5,5'-二硫代双(2-硝基苯甲酸)和N-乙基马来酰亚胺对巯基的修饰。

J Biol Chem. 1974 Jun 25;249(12):3916-22.

Nonfunctional nature of sulfhydryl groups for pigeon liver malic enzyme.

Int J Pept Protein Res. 1980 Oct;16(4):321-6. doi: 10.1111/j.1399-3011.1980.tb02593.x.

The substrate analog bromopyruvate as a substrate, an inhibitor and an alkylating agent of malic enzyme of pigeon liver.

Biochem Biophys Res Commun. 1973 Dec 10;55(3):580-7. doi: 10.1016/0006-291x(73)91183-2.

The pyruvate-proton exchange reaction of malic enzyme from pigeon liver.

Biochim Biophys Acta. 1982 Mar 18;702(1):54-60. doi: 10.1016/0167-4838(82)90026-7.

Determination of dissociation constants for enzyme-reactant complexes for NAD-malic enzyme by modulation of the thiol inactivation rate.通过调节硫醇失活速率测定NAD-苹果酸酶的酶-反应物复合物的解离常数。

Biochemistry. 1984 Nov 6;23(23):5454-9. doi: 10.1021/bi00318a012.

Equilibrium substrate binding studies of the malic enzyme of pigeon liver. Equivalence of nucleotide sites and anticooperativity associated with the binding of L-malate to the enzyme-manganese(II)-reduced nicotinamide adenine dinucleotide phosphate ternary complex.鸽肝苹果酸酶的平衡底物结合研究。L-苹果酸与酶-锰（II）-还原型烟酰胺腺嘌呤二核苷酸磷酸三元复合物结合相关的核苷酸位点等效性和负协同性。

Biochemistry. 1980 Mar 4;19(5):951-62. doi: 10.1021/bi00546a020.

Studies on regulatory functions of malic enzymes. VII. Structural and functional characteristics of sulfhydryl groups in NADP-linked malic enzyme from Escherichia coli W.苹果酸酶调节功能的研究。VII. 来自大肠杆菌W的NADP连接苹果酸酶中巯基的结构和功能特性。

J Biochem. 1979 Nov;86(5):1239-49. doi: 10.1093/oxfordjournals.jbchem.a132639.

Mechanism of pigeon liver malic enzyme modification of histidyl residues by ethoxyformic anhydride.乙氧基甲酸酐对鸽肝苹果酸酶组氨酸残基的修饰机制。

Biochim Biophys Acta. 1977 Aug 11;483(2):228-35. doi: 10.1016/0005-2744(77)90051-1.

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Evidence for the Existence of Two Essential and Proximal Cysteinyl Residues in NADP-Malic Enzyme from Maize Leaves.证据表明玉米叶片中的 NADP-苹果酸酶存在两个必需且紧邻的半胱氨酸残基。

Plant Physiol. 1992 Dec;100(4):2035-40. doi: 10.1104/pp.100.4.2035.

Pigeon liver malic enzyme.鸽肝苹果酸酶

Mol Cell Biochem. 1982 Mar 5;43(1):3-26. doi: 10.1007/BF00229535.

Reversible dissociation of the catalytically active subunits of pigeon liver malic enzyme.鸽肝苹果酸酶催化活性亚基的可逆解离

Biochem J. 1988 Aug 15;254(1):123-30. doi: 10.1042/bj2540123.

Duck liver 'malic' enzyme. Expression in Escherichia coli and characterization of the wild-type enzyme and site-directed mutants.鸭肝“苹果酸”酶。在大肠杆菌中的表达以及野生型酶和定点突变体的特性

Biochem J. 1992 Jun 15;284 ( Pt 3)(Pt 3):869-76. doi: 10.1042/bj2840869.

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