Persson H, Katze M G, Philipson L
J Virol. 1981 Nov;40(2):358-66. doi: 10.1128/JVI.40.2.358-366.1981.
We studied the accumulation of viral mRNA in the presence of inhibitors of protein synthesis in an adenovirus type 5-transformed cell line (line 293 cells). An analysis of the endogenous viral mRNA's and proteins revealed that only early regions 1A and 1B were expressed in uninfected 293 cells. However, viral mRNA's from early regions 2, 3, and 4, as well as mRNA's from early regions 1A and 1B, accumulated in 293 cells after infection with adenovirus type 2. Cells treated with anisomycin before infection showed a drastic enhancement of mRNA from early region 4 compared with drug-free controls, This increase in viral mRNA was detected by using filter hybridization, S1 endonuclease mapping, and in vitro translation. The rate of transcription of early region 4 nuclear RNA also increased significantly in the presence of anisomycin. In contrast to the results with early region 4, the levels of cytoplasmic mRNA's from early regions 2 and 3 did not increase in cells treated with inhibitors. These results suggested that multiple virus encoded controls operate on the early regions of the adenovirus genome.
我们在一种5型腺病毒转化的细胞系（293细胞系）中，研究了在蛋白质合成抑制剂存在的情况下病毒mRNA的积累情况。对内源性病毒mRNA和蛋白质的分析表明，在未感染的293细胞中仅表达早期区域1A和1B。然而，在293细胞感染2型腺病毒后，来自早期区域2、3和4的病毒mRNA，以及来自早期区域1A和1B的mRNA均会积累。在感染前用茴香霉素处理的细胞，与未用药物处理的对照相比，早期区域4的mRNA显著增加。通过滤膜杂交、S1核酸酶图谱分析和体外翻译检测到了病毒mRNA的这种增加。在茴香霉素存在的情况下，早期区域4核RNA的转录速率也显著增加。与早期区域4的结果相反，在用抑制剂处理的细胞中，来自早期区域2和3的细胞质mRNA水平并未增加。这些结果表明，多种病毒编码的调控作用于腺病毒基因组的早期区域。
