一项关于内源性核酸水解反应以及微球菌核酸酶和脱氧核糖核酸酶I对盐溶性紧密染色质形式作用的研究。
A study of an endogenous nucleolytic reaction and of the action micrococcal nuclease and DNAase I on a salt-soluble, compact form of chromatin.
作者信息
Krueger R C
出版信息
Biochim Biophys Acta. 1978 Sep 27;520(2):358-67. doi: 10.1016/0005-2787(78)90233-2.
The endogenous nucleolytic reaction occurring in rabbit thymus nuclear lysates has been studied at extended incubation times (up to 4 h). Production of nucleosomal polymers containing multiples of 205 base pairs of DNA was observed. The stability of the bands and the low release (1%) of acid-soluble nucleotides indicated there was only a small fraction of sensitive DNA between the subunits. The salt-soluble chromatin formed in the endogenous reaction at short incubation times (14--24 min) and purified over Sephadex G-200 has been treated with micrococcal nuclease and DNAase I. With micrococcal nuclease, nucleosomal polymers containing multiples of 201 base pairs of DNA were formed. Extensive digestion reaveled a core subunit containing 145 base pairs of DNA. With DNAase I only random degradation was observed and nucleosomal complexes were not produced.
我们对兔胸腺核裂解物中发生的内源性核酸水解反应进行了长达4小时的延长孵育时间研究。观察到产生了含有205个碱基对倍数的核小体聚合物。条带的稳定性以及酸溶性核苷酸的低释放率（1%）表明亚基之间只有一小部分敏感DNA。在短孵育时间（14 - 24分钟）内发生的内源性反应中形成并通过葡聚糖凝胶G - 200纯化的盐溶性染色质，已用微球菌核酸酶和DNA酶I处理。用微球菌核酸酶处理后，形成了含有201个碱基对倍数的核小体聚合物。广泛消化揭示了一个含有145个碱基对DNA的核心亚基。用DNA酶I处理时，仅观察到随机降解，未产生核小体复合物。
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