核小体之间及内部的核酸酶消化。

Greil W, Igo-Kemenes T, Zachau H G

Nucleic Acids Res. 1976 Oct;3(10):2633-44. doi: 10.1093/nar/3.10.2633.

In the course of digestions of rat liver nuclei with micrococcal nuclease the size of the nucleosomal DNA is shortened by 50-60 nucleotide pairs from starting lengths of about 200, 400, 600, 800, etc. nucleotide pairs in the monomeric and oligomeric nucleosomes, respectively. Acid soluble DNA material is created relatively slowly as compared to the rate of formation of subnucleosomal material. More DNA with lengths in between the 200, 400, etc. nucleotide pairs of nucleosomal DNA is formed when digestions with micrococcal nuclease are carried out at 0 to 10 degrees C compared to 40 degrees C. With DNAase II, on the other hand, formation of a 200 nucleotide pair pattern is favoured at the low temperatures. Apparently, the accessibility of potential cleavage sites in between and within nucleosomes depends strongly on the conditions of digestion. Possible reasons for this dependence are discussed.

在用微球菌核酸酶消化大鼠肝细胞核的过程中，核小体DNA的大小从单体和寡聚核小体中分别约200、400、600、800等核苷酸对的起始长度缩短了50 - 60个核苷酸对。与亚核小体物质的形成速率相比，酸溶性DNA物质的产生相对较慢。与40℃相比，在0至10℃下用微球菌核酸酶消化时，会形成更多长度介于核小体DNA的200、400等核苷酸对之间的DNA。另一方面，对于脱氧核糖核酸酶II，在低温下有利于形成200核苷酸对的模式。显然，核小体之间和内部潜在切割位点的可及性在很大程度上取决于消化条件。讨论了这种依赖性的可能原因。