用脱氧核糖核酸酶II消化染色质。
Digestion of chromatin with deoxyribonuclease II.
作者信息
Yaneva M, Dessev G
出版信息
Mol Biol Rep. 1977 Mar;3(3):227-34. doi: 10.1007/BF00643478.
PMID:870818
Abstract
The action of DNAse II on DNA in chromatin was studied. The formation of acid-soluble products followed a two-phase kinetic curve. At the end of the first more rapid phase about 25% of DNA was degraded. Early in the degradation process DNA was converted into double stranded fragments, whose sizes were multiples of about 180 base pairs. As the degradation proceeded these fragments were reduced in size. After denaturation DNA from digested chromatin was resolved into discrete single stranded fractions, exact multiples of a ten-nucleotide length, forming a pattern very similar to that observed with DNAase I.chkova LV
摘要
研究了脱氧核糖核酸酶II对染色质中DNA的作用。酸溶性产物的形成遵循两相动力学曲线。在第一个较快阶段结束时，约25%的DNA被降解。在降解过程早期，DNA被转化为双链片段，其大小为约180个碱基对的倍数。随着降解的进行，这些片段的大小减小。消化后的染色质中的DNA变性后被分解为离散的单链片段，其长度为十个核苷酸的精确倍数，形成的模式与脱氧核糖核酸酶I观察到的非常相似。奇科娃LV
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本文引用的文献
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Internal structure of the chromatin subunit.染色质亚基的内部结构。
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Interphase chromosomal deoxyribonucleoprotein isolated as a discrete structure from cultured cells.从培养细胞中分离出的作为离散结构的间期染色体脱氧核糖核蛋白。
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