丙二醛和乙醛对培养的哺乳动物细胞的影响。微核及染色体畸变的产生。

Effect of malonaldehyde and acetaldehyde on cultured mammalian cells. Production of micronuclei and chromosomal aberrations.

作者信息

Bird R P, Draper H H, Basrur P K

出版信息

Mutat Res. 1982 May;101(3):237-46. doi: 10.1016/0165-1218(82)90155-0.

Abstract

The genotoxic effects of malonaldehyde (MA) and acetaldehyde (AA) were investigated using primary cultures of rat skin fibroblasts. Exposure to MA at 10(-4) to 10(-3) M concentrations resulted in dose-dependent production of micronuclei. MA was approx. 10 times as potent as AA with respect to micronuclei formation. Treatment with 10(-4) and 10(-3) M concentrations of MA for 12 h produced chromosomal aberrations (chromosomal fragments, achromatic lesions and chromatid breaks) in 14 and 34% of metaphases, respectively. At 24 h the corresponding frequencies were 46 and 52%. AA at analogous concentrations produced aberrations in 4 and 14% of metaphases at 12 h, and 20 and 40% at 24 h. Dose-dependent increases in aneuploidy were seen at 10(-4) M and higher concentrations of both aldehydes, with incidences twice as high for MA as for AA.

摘要

利用大鼠皮肤成纤维细胞原代培养物研究了丙二醛（MA）和乙醛（AA）的遗传毒性作用。暴露于浓度为10⁻⁴至10⁻³ M的MA会导致微核的产生呈剂量依赖性。就微核形成而言，MA的效力约为AA的10倍。用10⁻⁴和10⁻³ M浓度的MA处理12小时，分别在14%和34%的中期产生了染色体畸变（染色体片段、无色病变和染色单体断裂）。在24小时时，相应的频率分别为46%和52%。类似浓度的AA在12小时时在4%和14%的中期产生畸变，在24小时时为20%和40%。在10⁻⁴ M及以上浓度的两种醛类中均观察到非整倍体的剂量依赖性增加，MA的发生率是AA的两倍。