Lewis A
Biophys J. 1978 Oct;24(1):249-54. doi: 10.1016/S0006-3495(78)85366-1.
Resonance Raman data on bathorhodopsin (bovine and squid) at 95,77, and 4 degrees K support a mechanism of excitation proposed by Lewis in which both a protein conformational transition and chromophore structural alteration to a "dicisoid" configuration are required to generate the bathorhodopsin species observed in steady-state photostationary mixtures. However, these results also suggest that the molecular structure with a red-shifted chromophore absorption detected at room temperatures in 1 ps using picosecond absorption spectroscopy may not necessarily have the same chromophore conformation as the steady-state bathorhodopsin species.
在95K、77K和4K温度下对牛和鱿鱼视紫红质中间体的共振拉曼数据支持了刘易斯提出的激发机制，即在该机制中，蛋白质构象转变和发色团结构改变为“双盘状”构型，都是产生稳态光稳定混合物中观察到的视紫红质中间体所必需的。然而，这些结果也表明，利用皮秒吸收光谱在室温下1皮秒内检测到的具有红移发色团吸收的分子结构，其发色团构象不一定与稳态视紫红质中间体相同。
