Burwen S J
Tissue Cell. 1982;14(1):125-34. doi: 10.1016/0040-8166(82)90012-x.
Mature mast cells, isolated from the rat peritoneal cavity, were placed into suspension culture, either as resting or after degranulation by exposure to compound 48/80, and were maintained for up to 63 hr. No mitotic cells were observed, and cell number was conserved. The culture conditions did not cause spontaneous degranulation and cell survival was better than 80%. However, with time in culture, an increasing percentage of cells acquired a vesiculated appearance, characterized by a Golgi area with distended cisternae, the accumulation of lysosomal or autophagic-like vesicles, and enlarged, irregular or fused secretory granules. In the degranulated group, about one-fourth of the cells recovered the morphological appearance of resting cells by 63 hr, indicating that they are capable of 'recycling'. A cell type with a unique morphology, characterized by a large central vacuole containing secretory product, an eccentric nucleus, and mature secretory granules at the cell periphery appeared in the stimulated group after 22 hr of culture. In may be a possible intermediate stage in the mast cell regranulation process, based on its occurrence exclusively in the stimulated group, the correlation between its distribution and the recovery of mast cells to the resting state, and the morphological resemblance of its granule contents to stages in granule maturation in differentiating embryonic mast cells.
从大鼠腹腔分离出的成熟肥大细胞，以静置状态或经化合物48/80刺激脱颗粒后进行悬浮培养，培养长达63小时。未观察到有丝分裂细胞，细胞数量保持稳定。培养条件未导致自发脱颗粒，细胞存活率高于80%。然而，随着培养时间的延长，出现囊泡样外观的细胞百分比逐渐增加，其特征为高尔基体区域的扁平囊泡扩张、溶酶体或自噬样囊泡积累以及分泌颗粒增大、不规则或融合。在脱颗粒组中，约四分之一的细胞在63小时时恢复到静置细胞的形态外观，表明它们能够“循环利用”。培养22小时后，在刺激组中出现了一种具有独特形态的细胞类型，其特征为含有分泌产物的大中央液泡、偏心核以及细胞周边的成熟分泌颗粒。基于其仅在刺激组中出现、其分布与肥大细胞恢复到静止状态之间的相关性以及其颗粒内容物与分化胚胎肥大细胞颗粒成熟阶段的形态相似性，它可能是肥大细胞再颗粒化过程中的一个可能中间阶段。
