Kayahara T
Tissue Cell. 1982;14(1):171-81. doi: 10.1016/0040-8166(82)90016-7.
The central parts of the chick notochord at Hamburger and Hamilton's stages 20-22 were investigated by electron microscopy. Electron-dense bodies of various sizes and shapes and bounded by a limiting membrane were found in the central cells the notochord. These dense bodies contained fibrous material or microfibrils which ranged from 120 to 600 A in diameter. The large microfibrils often exhibited a typical repeating period with an interval of about 320 A. These dense bodies were always located near the cell membrane, which is rough or irregular in the central parts of the notochord at these stages. The fibrous core material of the dense body frequently shows striking similarities to amorphous fibrous material in the intercellular space of the central parts of the notochord, where they are situated at a considerable distance from the perinotochordal sheath space. From these results, it seems reasonable to suggest that the central cells as well as the peripheral cells of the notochord are capable of forming microfibrils similar to those observed in the perinotochordal sheath space. Moreover, they may play an important role in the total fibrillogenesis of the notochord.
通过电子显微镜对处于汉伯格和汉密尔顿20 - 22期的鸡胚脊索中央部分进行了研究。在脊索的中央细胞中发现了各种大小和形状、由一层限制膜包被的电子致密体。这些致密体含有直径在120至600埃之间的纤维状物质或微原纤维。大型微原纤维常呈现出约320埃间隔的典型重复周期。这些致密体总是位于细胞膜附近，在这些阶段脊索中央部分的细胞膜粗糙或不规则。致密体的纤维状核心物质常常与脊索中央部分细胞间空间中的无定形纤维状物质有显著相似性，在那里它们与脊索周鞘空间相距甚远。从这些结果来看，认为脊索的中央细胞以及外周细胞都能够形成与在脊索周鞘空间中观察到的微原纤维相似的微原纤维似乎是合理的。此外，它们可能在脊索的整体纤维形成过程中发挥重要作用。
