大鼠肝微粒体对芘的代谢及各种单加氧酶诱导剂的影响。
The metabolism of pyrene by rat liver microsomes and the influence of various mono-oxygenase inducers.
作者信息
Jacob J, Grimmer G, Raab G, Schmoldt A
出版信息
Xenobiotica. 1982 Jan;12(1):45-53. doi: 10.3109/00498258209052453.
PMID:7090418
Abstract
- Pyrene metabolite g.l.c. profiles were recorded and metabolites identified by mass spectrometry. 2. Pyrene is metabolized by liver microsomes of untreated rats to 1-hydroxypyrene, 4,5-dihydroxy-4,5-dihydropyrene, two different diphenols and a triol, tentatively identified as 1,4,5-trihydroxy-4,5-dihydropyrene. 3. Pretreatment with phenobarbital or polychlorinated biphenyls favours oxidation at the K-region, whereas cytochrome P-448 inducers stimulate oxidation at the non-K-region of pyrene. 4. 1-Hydroxypyrene does not inhibit pyrene oxidation. 5. Pyrene diphenols are formed by secondary oxidation of 1-hydroxypyrene. 6. Triols are formed from dihydrodiols by secondary oxidation.
摘要
- 记录了芘代谢物的气相色谱图，并通过质谱法鉴定了代谢物。2. 芘在未处理大鼠的肝微粒体中代谢为1-羟基芘、4,5-二羟基-4,5-二氢芘、两种不同的二酚和一种三醇，初步鉴定为1,4,5-三羟基-4,5-二氢芘。3. 用苯巴比妥或多氯联苯预处理有利于芘在K区域的氧化，而细胞色素P-448诱导剂则刺激芘在非K区域的氧化。4. 1-羟基芘不抑制芘的氧化。5. 芘二酚是由1-羟基芘的二次氧化形成的。6. 三醇是由二氢二醇通过二次氧化形成的。
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