Butler J A, Whanger P D, Tripp M J
Am J Clin Nutr. 1982 Jul;36(1):15-23. doi: 10.1093/ajcn/36.1.15.
The influence of pregnancy on blood selenium levels and glutathione peroxidase (GSH-Px) activity was studied in women. Whole blood and plasma selenium levels decreased whereas erythrocyte and plasma GSH-Px activities increased with the progress of pregnancy. The ratio of erythrocyte GSH-Px activity to whole blood selenium levels was 4- to 5-fold higher in rats and sheep than in primates (humans and monkeys), suggesting that more selenium is associated with GSH-Px in erythrocytes from rats and sheep than from primates. In assays of blood with low GSH-Px activity such as that from humans or selenium-deficient animals, a component of the erythrocyte other than GSH-Px was found to contribute more to the peroxidase activity. Evidence was obtained to indicate that t-butyl hydroperoxide is a better substrate than hydrogen peroxide for the assay of low GSH-Px erythrocyte activity. The length of time that the blood was stored before assay was found to have an effect on erythrocyte GSH-Px activity, and the storage patterns may be dependent on the species of animal from which the blood is drawn.
研究了妊娠对女性血液硒水平和谷胱甘肽过氧化物酶（GSH-Px）活性的影响。随着妊娠进展，全血和血浆硒水平下降，而红细胞和血浆GSH-Px活性增加。大鼠和绵羊红细胞GSH-Px活性与全血硒水平的比值比灵长类动物（人类和猴子）高4至5倍，这表明大鼠和绵羊红细胞中与GSH-Px结合的硒比灵长类动物更多。在对GSH-Px活性较低的血液（如来自人类或缺硒动物的血液）进行检测时，发现红细胞中除GSH-Px外的其他成分对过氧化物酶活性的贡献更大。有证据表明，叔丁基过氧化氢比过氧化氢更适合用于检测低GSH-Px活性的红细胞。发现检测前血液储存的时间长度对红细胞GSH-Px活性有影响，且储存模式可能取决于采血动物的种类。
