Kanaide H, Yoshimura R, Makino N, Nakamura M
Am J Physiol. 1982 Jun;242(6):H980-9. doi: 10.1152/ajpheart.1982.242.6.H980.
Regional changes in myocardial function and oxidative metabolism during acute coronary artery occlusion were recorded spectrophotometrically by incorporating fiber optics in the isolated rat heart perfused by Langendorff's procedure. Oxygen saturation of myoglobin, reduction of cytochrome aa3, and the dynamic wall thickness of the left ventricle were continuously and concurrently measured from absorbancy increments at 581-592 nm, 605-630 nm, and 568-592 nm, respectively. In contrast to a gradual decrease in the extent of systolic wall thickening in anoxia, observed decreases in both the extent and the duration of systolic wall thickening and the appearance of a late systolic bulge occurred within 5 s after the onset of regional ischemia. After 10 s of both anoxia and regional ischemia, oxygen saturation of myoglobin decreased by 50%, but fluorescence of nicotinamide adenine dinucleotide remained at aerobic level which indicated that mitochondrial oxidative energy production might still be maintained. Thus early and pronounced dysfunction of the ischemic region appeared to precede a substantial loss of ATP production.
通过将光纤并入采用Langendorff灌流法的离体大鼠心脏，用分光光度法记录急性冠状动脉闭塞期间心肌功能和氧化代谢的区域变化。分别从581 - 592nm、605 - 630nm和568 - 592nm处吸光度的增加，连续同时测量肌红蛋白的氧饱和度、细胞色素aa3的还原以及左心室的动态壁厚度。与缺氧时收缩期壁增厚程度逐渐降低相反，在局部缺血开始后5秒内，观察到收缩期壁增厚的程度和持续时间均降低，并且出现了收缩期末期膨出。在缺氧和局部缺血10秒后，肌红蛋白的氧饱和度下降了50%，但烟酰胺腺嘌呤二核苷酸的荧光仍保持在需氧水平，这表明线粒体氧化能量产生可能仍得以维持。因此，缺血区域早期明显的功能障碍似乎先于ATP生成的大量损失。
