正常大鼠肺泡毛细血管膜及肺泡内衬物质中自体白蛋白的超微结构免疫过氧化物酶显示
Ultrastructural immunoperoxidase demonstration of autologous albumin in the alveolar capillary membrane and in the alveolar lining material in normal rats.
作者信息
Bignon J, Chahinian P, Feldmann G, Sapin C
出版信息
J Cell Biol. 1975 Feb;64(2):503-9. doi: 10.1083/jcb.64.2.503.
Abstract
The location of autologous serum albumin within the alveolar-capillary membrane was studied in the rat under physiological conditions using antialbumin antibodies labeled with peroxidase. Albumin was detected in the lung interstitium, and in numerous pinocytic vesicles within endothelial cells and type I alveolar epithelial cells. The immunoreaction was also positive at the level of plasmalemmal membranes of both cell types and in the alveolar lining material.
摘要
在生理条件下，利用过氧化物酶标记的抗白蛋白抗体，在大鼠体内研究了自体血清白蛋白在肺泡-毛细血管膜内的位置。在肺间质、内皮细胞和I型肺泡上皮细胞内的许多吞饮小泡中检测到白蛋白。在两种细胞类型的质膜水平以及肺泡内衬物质中，免疫反应也呈阳性。
相似文献
1
Ultrastructural immunoperoxidase demonstration of autologous albumin in the alveolar capillary membrane and in the alveolar lining material in normal rats.
J Cell Biol. 1975 Feb;64(2):503-9. doi: 10.1083/jcb.64.2.503.
2
Immunoelectron microscopic and immunochemical demonstrations of serum proteins in the alveolar lining material of the rat lung.
Am Rev Respir Dis. 1976 Feb;113(2):109-20. doi: 10.1164/arrd.1976.113.2.109.
3
Plasma membrane and internalized immunoglobulins of lymph node cells studied with conjugates of antibody or its Fab fragments with horseradish peroxidase.
J Cell Biol. 1974 Oct;63(1):12-23. doi: 10.1083/jcb.63.1.12.
4
Ultrastructural study by immunoperoxidase of a rat membranous glomerulonephritis.
J Ultrastruct Res. 1975 Feb;50(2):150-8. doi: 10.1016/s0022-5320(75)80045-1.
5
Ultrastructural appearances of pulmonary capillaries at high transmural pressures.
J Appl Physiol (1985). 1991 Aug;71(2):573-82. doi: 10.1152/jappl.1991.71.2.573.
6
Ultrastructural organization of the alveolar-capillary unit.
Ciba Found Symp. 1980;78:11-36. doi: 10.1002/9780470720615.ch2.
7
Structural features of alveolar wall basement membrane in the adult rat lung.
J Cell Biol. 1981 Nov;91(2 Pt 1):427-37. doi: 10.1083/jcb.91.2.427.
8
Permeability of the blood-air barrier to antiperoxidase antibodies and their fragments in the normal rat lung.
Am Rev Respir Dis. 1982 Jun;125(6):734-9. doi: 10.1164/arrd.1982.125.6.734.
9
Effect of endocytosis inhibitors on alveolar clearance of albumin, immunoglobulin G, and SP-A in rabbits.
Am J Physiol. 1994 May;266(5 Pt 1):L544-52. doi: 10.1152/ajplung.1994.266.5.L544.
10
Pulmonary microcirculation: differences in endothelia of subpleural and alveolar capillaries.
Microvasc Res. 1986 Nov;32(3):348-58. doi: 10.1016/0026-2862(86)90070-1.
引用本文的文献
1
Immune complex-mediated lung injury produced by horseradish peroxidase (HRP) and anti-HRP antibodies in rats.
Am J Pathol. 1982 Apr;107(1):16-24.
2
The pulmonary extracellular lining.
Environ Health Perspect. 1984 Apr;55:227-37. doi: 10.1289/ehp.8455227.
3
Immunohistochemical techniques and their applications in the histopathology of the respiratory system.
Environ Health Perspect. 1984 Jun;56:131-48. doi: 10.1289/ehp.8456131.
4
Bronchoalveolar lavage proteins.
Klin Wochenschr. 1985 Sep 2;63(17):781-7. doi: 10.1007/BF01732281.
5
Protein permeability in the adult respiratory distress syndrome. Loss of size selectivity of the alveolar epithelium.
J Clin Invest. 1986 Dec;78(6):1513-22. doi: 10.1172/JCI112743.
6
Uptake and intracellular transport of cationic ferritin in the bronchiolar and alveolar epithelia of the rat.
Cell Tissue Res. 1992 May;268(2):335-40. doi: 10.1007/BF00318802.
7
Transendothelial transport of serum albumin: a quantitative immunocytochemical study.
J Cell Biol. 1992 May;117(4):745-55. doi: 10.1083/jcb.117.4.745.
8
Secretory cells of the peripheral pulmonary epithelium as targets for toxic agents.
Environ Health Perspect. 1976 Aug;16:147-56. doi: 10.1289/ehp.7616147.
9
The air-blood barrier in the human lung. A freeze-fracture study.
Cell Tissue Res. 1979 May 18;198(2):269-85. doi: 10.1007/BF00232010.
10
Induction of anti-glomerular basement membrane antibodies in the Brown-Norway rat by mercuric chloride.
Clin Exp Immunol. 1977 Apr;28(1):173-9.
本文引用的文献
1
The hydrolysis of rabbit y-globulin and antibodies with crystalline papain.
Biochem J. 1959 Sep;73(1):119-26. doi: 10.1042/bj0730119.
2
LYMPH-FLOW AND CLEARANCE OF LIQUID FROM THE LUNGS OF THE FOETAL LAMB.
Lancet. 1965 Sep 4;2(7410):473-4. doi: 10.1016/s0140-6736(65)91428-5.
3
PERMEABILITY OF THE ALVEOLAR MEMBRANE TO SOLUTES.
Circ Res. 1965 Apr;16:353-62. doi: 10.1161/01.res.16.4.353.
4
Absorption of radioactive albumin from the lungs of normal dogs.
J Lab Clin Med. 1963 Mar;61:494-500.
5
The ultrastructural basis of alveolar-capillary membrane permeability to peroxidase used as a tracer.
J Cell Biol. 1968 Jun;37(3):781-93. doi: 10.1083/jcb.37.3.781.
6
Structural modulations of plasmalemmal vesicles.
J Cell Biol. 1968 Jun;37(3):633-49. doi: 10.1083/jcb.37.3.633.
7
Clearances of plasma proteins from pulmonary vascular beds of adult dogs and pups.
Am J Physiol. 1967 Aug;213(2):441-9. doi: 10.1152/ajplegacy.1967.213.2.441.
8
The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.
J Histochem Cytochem. 1966 Apr;14(4):291-302. doi: 10.1177/14.4.291.
9
Permeability of the mammalian blood-gas barrier and its components.
Am J Physiol. 1969 Apr;216(4):719-27. doi: 10.1152/ajplegacy.1969.216.4.719.
10
Pulmonary lymphatic and blood absorption of albumin from alveoli. A quantitative comparison.
Lab Invest. 1969 Jan;20(1):1-8.
Zotero 插件