一种用于测定N-乙酰-β-D-氨基葡萄糖苷酶的简单自动化比色法。
A simple automated colorimetric method for determination of N-acetyl- beta-D-glucosaminidase.
作者信息
Moore J C, Morris J E
出版信息
Ann Clin Biochem. 1982 May;19(3):157-9. doi: 10.1177/000456328201900305.
An automated method for the determination of N-acetyl-beta-D-glucosaminidase in serum or plasma using p-nitrophenol (pNP) N-acetyl-beta-D-glucosamine as substrate and a Pye Unicam AURA system is described. Normal samples had activities of 853 +/- 146 (SD) nmol pNP liberated/ml/h, with intra-assay coefficient of variation 1.2% and inter-assay coefficient of variation 1.6%. Inhibition of enzyme activity by heparin in plasma samples can be reversed by the addition of calcium chloride to the buffer.
描述了一种使用对硝基苯酚（pNP）N-乙酰-β-D-葡萄糖胺作为底物和Pye Unicam AURA系统测定血清或血浆中N-乙酰-β-D-氨基葡萄糖苷酶的自动化方法。正常样本的活性为853±146（标准差）nmol对硝基苯酚释放/ml/小时，批内变异系数为1.2%，批间变异系数为1.6%。血浆样本中肝素对酶活性的抑制作用可通过向缓冲液中添加氯化钙来逆转。
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