Neveu P J, Perdoux D, Reiss D
Ann Immunol (Paris). 1982 Jan-Feb;133C(1):69-82. doi: 10.1016/0769-2625(82)90007-1.
Lymphocyte blastogenesis of guinea-pig spleen cells induced by various doses of phytohaemagglutinin (PHA), concanavalin A (conA) or lipopolysaccharide (LPS) was assayed in the absence or in the presence of normal guinea-pig serum, foetal calf serum or heterologous albumin or gamma-globulins. Opposite effects of diluted and of concentrated sera were observed on blastogenesis induced either by PHA or by ConA: high concentrations inhibited blastogenesis whereas low concentrations enhanced mitogenesis. Homologous serum inhibited LPS-induced blastogenesis whatever the concentration used. These results could be explained by the interaction of serum with the membrane of lymphocytes stimulated by ConA, PHA or LPS. Furthermore sera could interfere with ConA. The implications of these experiments are discussed.
在不存在或存在正常豚鼠血清、胎牛血清、异种白蛋白或γ球蛋白的情况下，检测了不同剂量的植物血凝素（PHA）、刀豆球蛋白A（ConA）或脂多糖（LPS）诱导的豚鼠脾细胞淋巴细胞增殖反应。观察到稀释血清和浓缩血清对PHA或ConA诱导的增殖反应有相反的作用：高浓度抑制增殖反应，而低浓度增强有丝分裂。无论使用何种浓度，同源血清均抑制LPS诱导的增殖反应。这些结果可以通过血清与ConA、PHA或LPS刺激的淋巴细胞膜的相互作用来解释。此外，血清可能会干扰ConA。讨论了这些实验的意义。
