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通过聚丙烯酰胺凝胶层中的分析等电聚焦法分离还原型 erabutoxin b 重折叠过程中的中间体。

Separation of intermediates in the refolding of reduced erabutoxin b by analytical isoelectric focusing in layers of polyacrylamide gel.

作者信息

Bouet F, Ménez A, Hider R C, Fromageot P

出版信息

Biochem J. 1982 Mar 1;201(3):495-9. doi: 10.1042/bj2010495.

DOI:10.1042/bj2010495
PMID:7092808
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1163674/
Abstract

Isoelectric focusing in a thin layer of polyacrylamide gel is shown to be a suitable method for the resolution of intermediates trapped during the refolding process of reduced cystine-containing proteins. The method has been applied to the well-characterized snake neurotoxin erabutoxin b. An explanation is offered for the relatively low rate of refolding of this polypeptide.

摘要

在聚丙烯酰胺凝胶薄层中进行等电聚焦被证明是一种适用于分离在含胱氨酸还原蛋白重折叠过程中截留的中间体的方法。该方法已应用于特性明确的蛇神经毒素—— erabutoxin b。文中对该多肽相对较低的重折叠速率给出了解释。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd56/1163674/2d91b1784e13/biochemj00382-0071-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd56/1163674/2d91b1784e13/biochemj00382-0071-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd56/1163674/2d91b1784e13/biochemj00382-0071-a.jpg

相似文献

1
Separation of intermediates in the refolding of reduced erabutoxin b by analytical isoelectric focusing in layers of polyacrylamide gel.通过聚丙烯酰胺凝胶层中的分析等电聚焦法分离还原型 erabutoxin b 重折叠过程中的中间体。
Biochem J. 1982 Mar 1;201(3):495-9. doi: 10.1042/bj2010495.
2
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本文引用的文献

1
Tritium labelling of the alpha-neurotoxin of Naja nigricollis.黑颈眼镜蛇α-神经毒素的氚标记
FEBS Lett. 1971 Oct 1;17(2):333-335. doi: 10.1016/0014-5793(71)80180-1.
2
Refolding of reduced short neurotoxins: circular dichroism analysis.还原型短神经毒素的重折叠：圆二色性分析
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The position of disulfide bonds in cobrotoxin.眼镜蛇毒素中二硫键的位置。
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4
The disulphide bonds of erabutoxin a, a neurotoxic protein of a sea-snake (Laticauda semifasciata) venom.海蛇（半环扁尾海蛇）毒液中的神经毒性蛋白—— erabutoxin a的二硫键
Biochem J. 1971 May;122(4):463-7. doi: 10.1042/bj1220463.
5
The amino acid sequences of erabutoxins, neurotoxic proteins of sea-snake (Laticauda semifasciata) venom.海蛇（半环扁尾海蛇）毒液中的神经毒性蛋白—— erabutoxins的氨基酸序列。
Biochem J. 1971 May;122(4):453-61. doi: 10.1042/bj1220453.
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Intermediates in the refolding of reduced pancreatic trypsin inhibitor.
J Mol Biol. 1974 Aug 15;87(3):579-602. doi: 10.1016/0022-2836(74)90105-3.
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Structure of snake toxins and their affinity to the acetylcholine receptor of fish electric organ.蛇毒素的结构及其与鱼类电器官乙酰胆碱受体的亲和力。
Toxicon. 1977;15(6):477-88. doi: 10.1016/0041-0101(77)90098-8.
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Kinetics of refolding of reduced ribonuclease.还原型核糖核酸酶的重折叠动力学
J Mol Biol. 1977 Jun 25;113(2):329-41. doi: 10.1016/0022-2836(77)90145-0.
9
Conformation of snake toxic polypeptides studied by a method of prediction and circular dichroism.采用预测方法和圆二色性研究蛇毒多肽的构象。
Biochimie. 1978 Sep 4;60(5):505-16. doi: 10.1016/s0300-9084(78)80866-9.
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Snake toxin secondary structure predictions. Structure activity relationships.蛇毒素二级结构预测。构效关系。
J Mol Biol. 1977 Sep 15;115(2):177-93. doi: 10.1016/0022-2836(77)90095-x.