通过聚丙烯酰胺凝胶层中的分析等电聚焦法分离还原型 erabutoxin b 重折叠过程中的中间体。
Separation of intermediates in the refolding of reduced erabutoxin b by analytical isoelectric focusing in layers of polyacrylamide gel.
作者信息
Bouet F, Ménez A, Hider R C, Fromageot P
出版信息
Biochem J. 1982 Mar 1;201(3):495-9. doi: 10.1042/bj2010495.
Abstract
Isoelectric focusing in a thin layer of polyacrylamide gel is shown to be a suitable method for the resolution of intermediates trapped during the refolding process of reduced cystine-containing proteins. The method has been applied to the well-characterized snake neurotoxin erabutoxin b. An explanation is offered for the relatively low rate of refolding of this polypeptide.
摘要
在聚丙烯酰胺凝胶薄层中进行等电聚焦被证明是一种适用于分离在含胱氨酸还原蛋白重折叠过程中截留的中间体的方法。该方法已应用于特性明确的蛇神经毒素—— erabutoxin b。文中对该多肽相对较低的重折叠速率给出了解释。
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