海蛇（半环扁尾海蛇）毒液中的神经毒性蛋白—— erabutoxins的氨基酸序列。

Erabutoxin b was reduced, S-carboxymethylated and hydrolysed with trypsin. Seven tryptic fragments were isolated by column chromatography and paper electrophoresis. Some of the fragments were further hydrolysed with alpha-chymotrypsin, pepsin, Nagarse, Proctase A or Proctase B. The amino acid sequences of the fragment peptides were determined by subtractive Edman degradation. 2. From the tryptic digest of reduced, S-carboxymethylated and trifluoroacetylated erabutoxin b two fragments were isolated. From the amino acid composition of the fragments and from the terminal sequence studies on the reduced and S-carboxymethylated erabutoxin b, the sequence of the above seven tryptic fragments was elucidated. 3. The tryptic digestion of reduced and S-carboxymethylated erabutoxin a gave fragments, only one of which was different from the corresponding fragment from erabutoxin b. The amino acid sequence analysis of the fragment peptide showed that the only difference between erabutoxins a and b was that the former had asparagine and the latter had histidine at position 26.

将 erabutoxin b 还原、S-羧甲基化并经胰蛋白酶水解。通过柱色谱和纸电泳分离出七个胰蛋白酶片段。部分片段再用α-糜蛋白酶、胃蛋白酶、纳加酶、蛋白酶 A 或蛋白酶 B 进一步水解。片段肽的氨基酸序列通过减法 Edman 降解法测定。2. 从还原、S-羧甲基化和三氟乙酰化的 erabutoxin b 的胰蛋白酶消化物中分离出两个片段。根据片段的氨基酸组成以及对还原和 S-羧甲基化的 erabutoxin b 的末端序列研究，阐明了上述七个胰蛋白酶片段的序列。3. 还原和 S-羧甲基化的 erabutoxin a 的胰蛋白酶消化产生了片段，其中只有一个片段与 erabutoxin b 的相应片段不同。片段肽的氨基酸序列分析表明，erabutoxins a 和 b 之间的唯一差异在于前者在第 26 位有天冬酰胺，而后者在该位置有组氨酸。

The amino acid sequences of erabutoxins, neurotoxic proteins of sea-snake (Laticauda semifasciata) venom.