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海蛇（半环扁尾海蛇）毒液中的神经毒性蛋白—— erabutoxin a的二硫键

The disulphide bonds of erabutoxin a, a neurotoxic protein of a sea-snake (Laticauda semifasciata) venom.

作者信息

Endo Y, Sato S, Ishii S, Tamiya N

出版信息

Biochem J. 1971 May;122(4):463-7. doi: 10.1042/bj1220463.

DOI:10.1042/bj1220463
PMID:5166329
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1176802/
Abstract

Erabutoxin a was partially hydrolysed with enzymes and sulphuric acid and the resulting peptides were separated from each other by column chromatography and paper electrophoresis. From the results of amino acid analyses of the sulphur-containing peptides and their oxidized components, all four disulphide bridges in the toxin molecule were located. The disulphide bonds were found between half-cystine residues at positions 3 and 24, 17 and 41, 43 and 54, and 55 and 60 from the N-terminus.

摘要

erabutoxin a用酶和硫酸进行部分水解，然后通过柱色谱法和纸电泳法将所得肽彼此分离。根据含硫肽及其氧化成分的氨基酸分析结果，确定了毒素分子中的所有四个二硫键。发现二硫键存在于从N端起第3和24、17和41、43和54以及55和60位的半胱氨酸残基之间。

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本文引用的文献

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CRYSTALLIZATION AND PROPERTIES OF COBROTOXIN FROM FORMOSAN COBRA VENOM.台湾眼镜蛇毒中眼镜蛇毒素的结晶及性质
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Multiple dipping procedures in paper chromatography: a specific test for hydroxy-proline.纸色谱法中的多次浸渍程序：羟脯氨酸的特异性检测
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[Specific color reactions of arginine and of tyrosine in paper chromatography].[精氨酸和酪氨酸在纸色谱法中的特定颜色反应]
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Colour reactions on paper chromatograms by a dipping technique.采用浸渍技术在纸色谱上进行颜色反应。
Nature. 1953 Jan 3;171(4340):43-4. doi: 10.1038/171043a0.
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Location of disulphide bridges by diagonal paper electrophoresis. The disulphide bridges of bovine chymotrypsinogen A.通过对角线纸电泳确定二硫键的位置。牛胰凝乳蛋白酶原A的二硫键
Biochem J. 1966 Oct;101(1):214-28. doi: 10.1042/bj1010214.
7
Studies on sea-snake venoms. Crystallization of erabutoxins a and b from Laticauda semifasciata venom.海蛇毒液研究。从半环扁尾海蛇毒液中结晶出 erabutoxins a 和 b。
Biochem J. 1966 Jun;99(3):624-30. doi: 10.1042/bj0990624.
8
Properties and biosynthesis of a neurotoxic protein of the venoms of s snakes Laticauda laticaudata and Laticauda colubrina.蓝灰扁尾海蛇和扁尾海蛇毒液中一种神经毒性蛋白的性质与生物合成
Biochem J. 1969 Oct;115(1):85-90. doi: 10.1042/bj1150085.
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The amino acid sequences of erabutoxins, neurotoxic proteins of sea-snake (Laticauda semifasciata) venom.海蛇（半环扁尾海蛇）毒液中的神经毒性蛋白—— erabutoxins的氨基酸序列。
Biochem J. 1971 May;122(4):453-61. doi: 10.1042/bj1220453.
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A neurotoxin, toxin alpha, from Egyptian cobra (Naja haje haje) venom. I. Purification, properties, and complete amino acid sequence.一种来自埃及眼镜蛇（Naja haje haje）毒液的神经毒素——毒素α。I. 纯化、特性及完整氨基酸序列。
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