海蛇（半环扁尾海蛇）毒液中的神经毒性蛋白—— erabutoxin a的二硫键
The disulphide bonds of erabutoxin a, a neurotoxic protein of a sea-snake (Laticauda semifasciata) venom.
作者信息
Endo Y, Sato S, Ishii S, Tamiya N
出版信息
Biochem J. 1971 May;122(4):463-7. doi: 10.1042/bj1220463.
Abstract
Erabutoxin a was partially hydrolysed with enzymes and sulphuric acid and the resulting peptides were separated from each other by column chromatography and paper electrophoresis. From the results of amino acid analyses of the sulphur-containing peptides and their oxidized components, all four disulphide bridges in the toxin molecule were located. The disulphide bonds were found between half-cystine residues at positions 3 and 24, 17 and 41, 43 and 54, and 55 and 60 from the N-terminus.
摘要
erabutoxin a用酶和硫酸进行部分水解，然后通过柱色谱法和纸电泳法将所得肽彼此分离。根据含硫肽及其氧化成分的氨基酸分析结果，确定了毒素分子中的所有四个二硫键。发现二硫键存在于从N端起第3和24、17和41、43和54以及55和60位的半胱氨酸残基之间。
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