Kasperek G J, Dohm G L, Barakat H A, Strausbauch P H, Barnes D W, Snider R D
Biochem J. 1982 Feb 15;202(2):281-8. doi: 10.1042/bj2020281.
Previous reports have shown that exercise causes a loss of liver protein. The purpose of the present study was to elucidate the mechanism of this exercise-induced protein loss. Exercise caused: (1) an increase in mechanical and osmotic lysosomal fragility; (2) a significant loss of hepatic water, glycogen, protein, phospholipid and RNA; (3) loss of protein from the soluble, mitochondrial and microsomal fractions: (4) loss of mitochondrial, microsomal and cytosolic, but not lysosomal, enzyme activity; (5) an increase in the number of autophagic vacuoles; (6) an increase in the lysosomal size. Taken together, these results suggest that the autophagolysosomal system is responsible for the exercise-induced hepatic protein loss.
先前的报告表明，运动导致肝脏蛋白质流失。本研究的目的是阐明这种运动诱导的蛋白质流失的机制。运动导致：（1）机械性和渗透性溶酶体脆性增加；（2）肝脏水分、糖原、蛋白质、磷脂和RNA显著流失；（3）可溶性、线粒体和微粒体部分的蛋白质流失；（4）线粒体、微粒体和胞质酶活性丧失，但溶酶体酶活性未丧失；（5）自噬泡数量增加；（6）溶酶体大小增加。综合这些结果表明，自噬溶酶体系统是运动诱导肝脏蛋白质流失的原因。
