White J O, Moore P A, Elder M G, Lim L
Biochem J. 1982 Feb 15;202(2):535-41. doi: 10.1042/bj2020535.
The effects of progesterone therapy (5 mg, administered subcutaneously daily for 6 days) on the abnormal uterus of adult anovulatory Wistar rats have been studied. These rats, rendered anovulatory by neonatal treatment with testosterone propionate or clomiphene citrate, displayed severe hyperplasia and metaplasia of the uterine luminal epithelium and a disproportionately high content of nuclear oestrogen receptor, as a result of constant oestrogen stimulation unrelieved by progesterone [White, Moore, Elder & Lim (1981) Biochem. J. 196, 557-565]. Progesterone therapy resulted in the virtual elimination of the hyperplasia and metaplasia and a corresponding decrease in the content of nuclear oestrogen receptor with the proportion of the unoccupied nuclear receptor being increased to values exhibited by normal cyclic females. There was also a decrease in the content of progestin receptors, a putative index of oestrogenic stimulation. Further, in the testosterone-treated group, progesterone therapy resulted in the restoration of oestrogen receptor translocational responses to oestradiol stimulation. Progesterone treatment of these anovulatory rats thus provides a model system for investigating the biochemical mechanisms underlying progestin antagonism and regulation of oestrogen-stimulated cell proliferation.
研究了孕酮疗法（5毫克，每日皮下注射，共6天）对成年无排卵Wistar大鼠异常子宫的影响。这些大鼠因新生期用丙酸睾酮或枸橼酸氯米芬处理而导致无排卵，由于持续的雌激素刺激未被孕酮缓解，其子宫腔上皮出现严重增生和化生，核雌激素受体含量异常高[怀特、摩尔、埃尔德和林（1981年）《生物化学杂志》196卷，557 - 565页]。孕酮疗法几乎消除了增生和化生，核雌激素受体含量相应降低，未占据的核受体比例增加到正常周期性雌性大鼠的水平。孕激素受体含量也有所下降，这是雌激素刺激的一个假定指标。此外，在睾酮处理组中，孕酮疗法导致雌激素受体对雌二醇刺激的转位反应恢复。因此，对这些无排卵大鼠进行孕酮治疗为研究孕激素拮抗作用和雌激素刺激细胞增殖调节的生化机制提供了一个模型系统。
