一种用于研究完整细胞中脱氢酶动力学特性的pmr同位素交换法。
A p.m.r. isotope-exchange method for studying the kinetic properties of dehydrogenases in intact cells.
作者信息
Simpson R J, Brindle K M, Brown F F, Campbell I D, Foxall D L
出版信息
Biochem J. 1982 Mar 15;202(3):573-9. doi: 10.1042/bj2020573.
Abstract
A method to determine the activity of dehydrogenases in an intact-cell system is described. The method involves the use of n.m.r. to monitor bulk isotope exchange. The approach is illustrated by application to the isotope equilibration of pyruvate and lactate as catalyzed by lactate dehydrogenase in intact erythrocytes. Particular problems peculiar to bulk isotope exchange and its observation by n.m.r. are considered.
摘要
本文描述了一种测定完整细胞系统中脱氢酶活性的方法。该方法涉及使用核磁共振（n.m.r.）来监测整体同位素交换。通过应用于完整红细胞中乳酸脱氢酶催化的丙酮酸和乳酸的同位素平衡来说明该方法。文中考虑了整体同位素交换及其通过核磁共振观察所特有的特殊问题。
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