Balk L, Meijer J, Bergstrand A, Aström A, Morgenstern R, Seidegård J, DePierre J W
Biochem Pharmacol. 1982 Apr 15;31(8):1491-500. doi: 10.1016/0006-2952(82)90371-9.
The present study was designed to prepare and characterize subcellular fractions from the liver of the Northern pike (Esox lucius), with special emphasis on the preparation of microsomal fractions suitable for studying xenobiotic metabolism. The purity of the different fractions obtained by differential centrifugation, as well as the recovery of different organelles, was determined using both enzyme markers and morphological examination with the electron microscope. Attempts were also made to increase the recovery of fragments of the endoplasmic reticulum in the microsomal fraction. Finally, the subcellular distribution of several drug-metabolizing enzymes (cytochrome P-450, benzpyrene monoxygenase, epoxide hydrolase and glutathione transferases) were determined. With the exception of the subcellular distribution of epoxide hydrolase, the results obtained here resemble closely those reported fo rat liver and the microsomal fraction prepared is highly suitable for further studies of drug metabolism in pike liver.
本研究旨在制备并表征白斑狗鱼（Esox lucius）肝脏的亚细胞组分，特别着重于制备适合研究外源性物质代谢的微粒体组分。通过差速离心获得的不同组分的纯度以及不同细胞器的回收率，使用酶标记物和电子显微镜形态学检查进行测定。还尝试提高微粒体组分中内质网片段的回收率。最后，测定了几种药物代谢酶（细胞色素P - 450、苯并芘单加氧酶、环氧化物水解酶和谷胱甘肽转移酶）的亚细胞分布。除环氧化物水解酶的亚细胞分布外，此处获得的结果与大鼠肝脏报道的结果非常相似，并且制备的微粒体组分非常适合进一步研究狗鱼肝脏中的药物代谢。
