Vrsanská M, Gorbacheva I V, Krátký Z, Biely P
Biochim Biophys Acta. 1982 May 21;704(1):114-22. doi: 10.1016/0167-4838(82)90138-8.
An acidic endo-1,4-beta-xylanase (1,4-beta-D-xylan xylanohydrolase, EC 3.2.1.8) of Aspergillus niger catalyzes degradation of linear 1,4-beta-xylooligosaccharides by multiple reaction pathways analogous to those catalyzed by lysozyme and alpha-amylases. Quantitative product analysis of enzyme-substrate mixtures using 1-3H-reducing end-labeled xylooligosaccharides and [U-14C]xylotriose led to the following conclusions: (1) bond cleavage frequencies of xylotriose, xylotetraose and xylopentaose are strongly dependent on substrate concentration; (2) at relatively low concentration of the oligosaccharides the enzyme catalyzes transglycosylic reactions leading to products larger than the substrates; (3) xylobiose and to a low extent also xylose, are utilized as glycosyl acceptors in the transfer reactions; (4) the enzyme-glycosyl intermediates effective in the transfer reactions are formed only from the non-reducing part of oligosaccharides, since no evidence was obtained for condensation of two molecules of oligosaccharides; (5) the enzyme does not catalyze degradation of xylobiose and aryl beta-xylosides at an appreciable rate.
黑曲霉的一种酸性内切 - 1,4 - β - 木聚糖酶（1,4 - β - D - 木聚糖木聚糖水解酶，EC 3.2.1.8）通过与溶菌酶和α - 淀粉酶催化的反应途径类似的多种反应途径催化线性1,4 - β - 木寡糖的降解。使用1 - ³H - 还原端标记的木寡糖和[U - ¹⁴C]木三糖对酶 - 底物混合物进行定量产物分析得出以下结论：（1）木三糖、木四糖和木五糖的键断裂频率强烈依赖于底物浓度；（2）在相对低浓度的寡糖条件下，该酶催化转糖基反应，产生比底物更大的产物；（3）木二糖以及在较低程度上木糖，在转移反应中用作糖基受体；（4）在转移反应中有效的酶 - 糖基中间体仅由寡糖的非还原部分形成，因为未获得两分子寡糖缩合的证据；（5）该酶不会以可观的速率催化木二糖和芳基β - 木糖苷的降解。
