McNamara D J, Proia A, Edwards K D
Biochim Biophys Acta. 1982 May 13;711(2):252-60. doi: 10.1016/0005-2760(82)90033-9.
The rate of whole bodyb cholesterol synthesis was measured in male Sprague-Dawley rats fed either a standard chow, cereal-based diet or a semi-synthetic purified diet consisting of casein, sucrose and lard. The purified diet significantly decreased daily fecal excretion of neutral and acidic sterols, the specific acitvity of hepatic 3-hydroxy-3-methylglutaryl coenzyme A reductase, the bile acid pool size, and total daily cholesterol synthesis in the rat, while increasing plasma cholesterol concentrations and the total body content of cholesterol. The increased body content of cholesterol occurred primarily in muscle and connective tissue and not in the liver. The data demonstrate the importance of quantitating the net tissue accumulatin of cholesterol for accurate measurement of daily sterol synthesis in growing animals when sterol balance measurements are used. Tissue accumulation accounted for 7% of total daily cholesterol synthesis in rats fed the cereal diet, and 20% of daily synthesis in animals fed the purified diet.
给雄性斯普拉格-道利大鼠喂食标准食物、谷类食物或由酪蛋白、蔗糖和猪油组成的半合成纯化食物，测量其全身胆固醇合成速率。纯化食物显著降低了大鼠中性和酸性固醇的每日粪便排泄量、肝脏3-羟基-3-甲基戊二酰辅酶A还原酶的比活性、胆汁酸池大小以及每日总胆固醇合成量，同时增加了血浆胆固醇浓度和全身胆固醇含量。胆固醇含量的增加主要发生在肌肉和结缔组织中，而非肝脏。这些数据表明，在使用固醇平衡测量法时，对于准确测量生长动物每日固醇合成量而言，量化胆固醇在组织中的净积累非常重要。在喂食谷类食物的大鼠中，组织积累占每日总胆固醇合成量的7%，在喂食纯化食物的动物中占每日合成量的20%。
