[Effect of cycloheximide on the alkaline and acid DNAse activity in rat cells].

Using spectrophotometric and viscosimetric techniques, the effects of cycloheximide on the activity of alkaline and acid DNAses in the homogenate cells of thymus, mesenterial lymphatic node, spleen, liver and in blood serum of the rat were studied. Using these two methods, it was demonstrated that cycloheximide inhibits the activity of alkaline DNAase in the spleen, lymphatic node and liver cells by 70% without changing that of acid DNAase in all the organs tested but has no inhibiting effect on the activity of alkaline endonuclease in the thymocytes. Cycloheximide inhibits the activity of alkaline DNAase in rat blood serum by 85%. It can thus be concluded that in the spleen, lymphatic node and liver cells there occurs a practically constant synthesis of alkaline DNAase, the bulk of which is released into the blood stream.