Elliott G F, Bartels E M
Biophys J. 1982 May;38(2):195-9. doi: 10.1016/S0006-3495(82)84546-3.
In this paper we reconsider the theoretical and practical aspects of using KCl-filled microelectrodes in extended polyelectrolyte gels such as muscle to measure Donnan potentials, and then calculate protein fixed-charge concentrations. An analytical calculation of the electrical potential function between muscle filaments shows that whether the microelectrode averages the ionic concentration or the local potentials the results are indistinguishable in the practical regime. After consideration of this and other possible sources of error, we conclude that the charge-concentrations measurements that have appeared in the literature are legitimate.
在本文中,我们重新审视了在诸如肌肉等延伸聚电解质凝胶中使用填充KCl的微电极来测量唐南电位,进而计算蛋白质固定电荷浓度的理论和实践方面。对肌丝间电势函数的解析计算表明,在实际情况下,无论微电极是对离子浓度还是局部电位进行平均,结果都是无法区分的。在考虑了这一因素以及其他可能的误差来源后,我们得出结论,文献中出现的电荷浓度测量结果是合理的。