Hoover D B, Barron S E
Brain Res Bull. 1982 Mar;8(3):279-84. doi: 10.1016/0361-9230(82)90060-0.
The acetylcholinesterase (AChE) content of rat vagal efferent neurons was studied. Retrograde transport of horseradish peroxidase (HRP) by cut vagal axons provided a means for localizing efferent cell bodies; tissue sections were then processed for the simultaneous visualization of HRP and AChE. A dorsal vagal efferent column contained the dorsal motor nucleus of the vagus, as a primary component, and extended caudally into the upper cervical spinal cord. A ventral column contained neurons in the nucleus ambiguus and the surrounding reticular formation. Although most of the vagal efferent neurons stained with moderate to heave intensity for AChE there were some HRP-labeled cells that contained little AChE and a small percentage in which AChE was absent. In spite of the fact that AChE has been demonstrated in certain non-cholinergic neurons, it has also been found in all cholinergic neurons. Therefore, the presence of AChE has been regarded as a necessary (but not sufficient) component for identifying cholinergic neurons. The absence of AChE in a small percentage of the vagal efferent neurons indicates that some preganglionic parasympathetic fibers in the vagus nerve are not cholinergic.
对大鼠迷走神经传出神经元的乙酰胆碱酯酶（AChE）含量进行了研究。切断的迷走神经轴突对辣根过氧化物酶（HRP）的逆行运输为定位传出细胞体提供了一种方法；然后对组织切片进行处理，以便同时观察HRP和AChE。迷走神经背侧传出柱以迷走神经背运动核为主要成分，并向尾侧延伸至上颈段脊髓。腹侧柱包含疑核和周围网状结构中的神经元。尽管大多数迷走神经传出神经元对AChE呈中度至重度染色，但仍有一些HRP标记的细胞AChE含量很少，还有一小部分细胞完全没有AChE。尽管已在某些非胆碱能神经元中证实存在AChE，但在所有胆碱能神经元中也都发现了AChE。因此，AChE的存在被视为识别胆碱能神经元的必要（但非充分）成分。一小部分迷走神经传出神经元中没有AChE，这表明迷走神经中的一些节前副交感神经纤维不是胆碱能的。
