Lai C N, Becker F F
Cancer Biochem Biophys. 1982;6(1):1-6.
Primary hepatocellular carcinoma(s) (PHC) induced in rats by N-2-fluorenylacetamide (AAF) or diethylnitrosamine (DEN) were examined for their bioelectric potential (EP). N-2-Fluorenylacetamide-induced PHC differed from age-matched normal livers by a mean of -12.9, which is significant at p less than 0.001. They also differed from non-PHC-bearing surrounding liver by -4.75 mV, significant at p less than 0.05. Diethylnitrosamine-induced PHC differed from normal liver by -13.5 mV and from background liver by -9.95 mV, both significant at p less than 0.001. To determine the contribution of cell division to these differences, regenerating livers were examined. The levels of mixed function oxidases in PHC and in regenerating livers reported in the literature appeared to correlate with the measured EP. We, therefore, studied the effects of chemical inhibitors and an inducer of this enzyme system. The alterations of mixed function oxidase were demonstrated to parallel those of EP.
对用N-2-芴基乙酰胺（AAF）或二乙基亚硝胺（DEN）诱导大鼠产生的原发性肝细胞癌（PHC）进行生物电位（EP）检测。N-2-芴基乙酰胺诱导的PHC与年龄匹配的正常肝脏相比，平均相差-12.9，在p小于0.001时具有显著性差异。它们与无PHC的周围肝脏相比也相差-4.75 mV，在p小于0.05时具有显著性差异。二乙基亚硝胺诱导的PHC与正常肝脏相差-13.5 mV，与背景肝脏相差-9.95 mV，两者在p小于0.001时均具有显著性差异。为了确定细胞分裂对这些差异的影响，对再生肝脏进行了检测。文献报道的PHC和再生肝脏中混合功能氧化酶的水平似乎与测得的EP相关。因此，我们研究了该酶系统的化学抑制剂和诱导剂的作用。结果表明，混合功能氧化酶的改变与EP的改变平行。
