钝顶螺旋藻中一种对乙硫氨酸具有抗性的突变体中，甲硫氨酰 - tRNA合成酶发生了改变。
Altered methionyl-tRNA synthetase in a Spirulina platensis mutant resistant to ethionine.
作者信息
Riccardi G, Sanangelantoni A M, Sarasini A, Ciferri O
出版信息
J Bacteriol. 1982 Aug;151(2):1053-5. doi: 10.1128/jb.151.2.1053-1055.1982.
Abstract
Compared with the parental strain, a Spirulina platensis mutant that is resistant to ethionine incorporated methionine into protein at a reduced rate, whereas ethionine incorporation was practically nil. The methionyl-tRNA synthetase present in crude extracts from the resistant strain showed a reduced affinity for methionine and ethionine.
摘要
与亲本菌株相比，钝顶螺旋藻的一个对乙硫氨酸有抗性的突变体将甲硫氨酸掺入蛋白质的速率降低，而乙硫氨酸的掺入实际上为零。来自抗性菌株的粗提物中存在的甲硫氨酰 - tRNA合成酶对甲硫氨酸和乙硫氨酸的亲和力降低。
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