一种用于诊断钩端螺旋体病的简单血清学方法的开发：微胶囊凝集试验。
Development of a simple serological method for diagnosing leptospirosis: a microcapsule agglutination test.
作者信息
Arimitsu Y, Kobayashi S, Akama K, Matuhasi T
出版信息
J Clin Microbiol. 1982 May;15(5):835-41. doi: 10.1128/jcm.15.5.835-841.1982.
Abstract
A passive microcapsule agglutination test for the diagnosis of leptospirosis was developed by utilizing chemically stable microcapsules instead of sheep erythrocytes. In the test, sonically disrupted antigens of leptospira were sensitized to microcapsules treated with glutaraldehyde. Compared with the microscopic agglutination test, the passive microcapsule agglutination test showed a relatively genus-specific tendency and a 4- to 32-fold-higher sensitivity. The sensitized microcapsule antigens were stable for at least 1 year. The microcapsules coupled with mixed antigens can be used as a serodiagnostic screening test for diseases caused by various types of leptospira. The test, which is very simple and reproducible and requiring no specific training, can be employed easily as a routine test in diagnostic laboratories.
摘要
利用化学性质稳定的微胶囊而非绵羊红细胞，开发了一种用于诊断钩端螺旋体病的被动微胶囊凝集试验。在该试验中，将经超声破碎的钩端螺旋体抗原致敏于用戊二醛处理过的微胶囊。与显微镜凝集试验相比，被动微胶囊凝集试验显示出相对属特异性的趋势，灵敏度高4至32倍。致敏的微胶囊抗原至少可稳定保存1年。与混合抗原偶联的微胶囊可作为针对由各种类型钩端螺旋体引起的疾病的血清学诊断筛查试验。该试验非常简单且可重复，不需要特殊培训，可轻松用作诊断实验室的常规试验。
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