Kobayashi S, Yamaya S I, Sugahara T, Matuhasi T
Br J Vener Dis. 1983 Feb;59(1):1-7. doi: 10.1136/sti.59.1.1.
For the serodiagnosis of syphilis a quantitative passive agglutination (MCA-TP) test for antibodies to Treponema pallidum was performed with chemically stable microcapsules with no antigenic activity instead of with conventional sheep erythrocytes. The microcapsules were easily sensitised with the antigen of sonicated Treponema pallidum by treatment with glutaraldehyde. Compared with the Treponema pallidum haemagglutination test (TPHA) the MCA-TP test was superior for detecting cases of primary syphilis. Furthermore, the decrease in antibody titre during treatment was more evident in this test than in the FTA-ABS or the TPHA tests. The MCA-TP test performed on IgM and IgG gel-filtered fractions of sera from patients with syphilis proved that the sensitised microcapsule antigen reacted sharply with the IgM antibodies specific to syphilis.
为进行梅毒的血清学诊断，采用化学性质稳定且无抗原活性的微胶囊替代传统绵羊红细胞，开展了针对梅毒螺旋体抗体的定量被动凝集试验（MCA - TP）。通过用戊二醛处理，微胶囊很容易被超声处理后的梅毒螺旋体抗原致敏。与梅毒螺旋体血凝试验（TPHA）相比，MCA - TP试验在检测一期梅毒病例方面更具优势。此外，在治疗过程中，该试验中抗体滴度的下降比荧光密螺旋体抗体吸收试验（FTA - ABS）或TPHA试验更为明显。对梅毒患者血清的IgM和IgG凝胶过滤组分进行的MCA - TP试验证明，致敏的微胶囊抗原与梅毒特异性IgM抗体反应强烈。
