Cacciapuoti B, Ciceroni L, Arimitsu Y, Sato T, Seki M
National Center for Leptospirosis, Istituto Superiore di Sanità, Rome, Italy.
Eur J Epidemiol. 1993 Jan;9(1):92-6. doi: 10.1007/BF00463096.
Microcapsules, absorbed with two mixed antigens--each composed of 3 sonicated leptospira serovars--were developed in the past as a microcapsule agglutination test (MCA-LS) for the screening of clinical leptospirosis. For this study, fifty serum samples, taken at an earlier and at a later stage of illness from 25 Italian in-patients with clinical symptoms of leptospirosis, were tested with both the MCA-LS one-dilution test and the microscopic agglutination (MA) test, the confirmatory test for leptospirosis, with 18 leptospira strains circulating in Italy. Compared with MA, MCA-LS showed a sensitivity of 91.7%, and a specificity of 92.3%, including leptospirosis not sustained by the diagnostic strains used in the MCA-LS kit.
微胶囊吸收了两种混合抗原，每种抗原由3种超声处理过的钩端螺旋体血清型组成，过去曾被开发为微胶囊凝集试验（MCA-LS）用于临床钩端螺旋体病的筛查。在本研究中，从25名有钩端螺旋体病临床症状的意大利住院患者疾病早期和晚期采集的50份血清样本，用MCA-LS单稀释试验和钩端螺旋体病确诊试验——显微镜凝集（MA）试验进行检测，MA试验使用在意大利流行的18种钩端螺旋体菌株。与MA试验相比，MCA-LS试验显示出91.7%的灵敏度和92.3%的特异性，包括MCA-LS试剂盒中所用诊断菌株未引起的钩端螺旋体病。
