Fine J B, Sprecher H
J Lipid Res. 1982 May;23(4):660-3.
A unidimensional thin-layer chromatography system for the separation of phospholipids has been developed. The method employs precoated plates impregnated with boric acid and is capable of resolving eight phospholipid standards, including phosphatidylinositol, phosphatidylserine, and phosphatidylglycerol. Liver, kidney, and platelet phospholipids of the rat were resolved into six major classes using this method. Each was judged to be pure by two-dimensional thin-layer chromatography. The use of boric acid thin-layer chromatography for the separation of phospholipids should find wide application.
已开发出一种用于分离磷脂的一维薄层色谱系统。该方法采用浸渍有硼酸的预涂板，能够分离出八种磷脂标准品，包括磷脂酰肌醇、磷脂酰丝氨酸和磷脂酰甘油。使用该方法可将大鼠的肝、肾和血小板磷脂分离为六大类。通过二维薄层色谱法判断每一类均为纯品。硼酸薄层色谱法在磷脂分离中的应用应会得到广泛应用。
