Poorthuis B J, Yazaki P J, Hostetler K Y
J Lipid Res. 1976 Jul;17(4):433-7.
A two dimensional thin-layer chromatography system has been devised for the improved separations of phosphatidylglycerol and its derivaties, cardiolipin and bis/monoacylglyceryl)phosphate, from the other phospholipid components of tissue total lipid extracts. The system employs silica gel G plates prepared with 0.4 M boric acid. Linear recovery of added phosphatidylglycerol was found, and phosphatidylglycerol did not cochromatograph with N, N-dimethylphosphatidylethanolamine in this system. The phospholipid class composition of various rat tissues and a Morris 7777 hepatoma has been determined and compared with values from the literature.
已设计出一种二维薄层色谱系统，用于从组织总脂质提取物的其他磷脂成分中更好地分离磷脂酰甘油及其衍生物、心磷脂和双/单酰基甘油磷酸酯。该系统采用用0.4M硼酸制备的硅胶G板。发现添加的磷脂酰甘油呈线性回收，且在该系统中磷脂酰甘油与N，N-二甲基磷脂酰乙醇胺不共色谱。已测定了各种大鼠组织和莫里斯7777肝癌的磷脂类组成，并与文献中的值进行了比较。
