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二维凝胶蛋白上一些噬菌体T4复制前蛋白的鉴定

Identification of some bacteriophage T4 prereplicative proteins on two-dimensional gel proteins.

作者信息

Cook K S, Seasholtz A F

出版信息

J Virol. 1982 Jun;42(3):767-72. doi: 10.1128/JVI.42.3.767-772.1982.

DOI:10.1128/JVI.42.3.767-772.1982
PMID:7097860
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC256909/
Abstract

Profiles of bacteriophage T4 early proteins resolved by a two-dimensional nonequilibrium pH gradient electrophoresis system (P. Z. O'Farrell, H. M. Goodman, and P. H. O'Farrell, Cell 12:1133--1142, 1977) are presented. Over 65 phage-induced proteins were resolved. Amber or deletion mutants were used to identify 17 proteins in the gel patterns as the products of specific genes.

摘要

本文展示了通过二维非平衡pH梯度电泳系统解析的噬菌体T4早期蛋白质图谱（P.Z.奥法雷尔、H.M.古德曼和P.H.奥法雷尔，《细胞》12:1133 - 1142，1977年）。分辨出了超过65种噬菌体诱导的蛋白质。利用琥珀突变体或缺失突变体在凝胶图谱中鉴定出17种蛋白质为特定基因的产物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3f1/256909/9b3f33255365/jvirol00159-0022-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3f1/256909/e64a623e6766/jvirol00159-0021-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3f1/256909/9b3f33255365/jvirol00159-0022-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3f1/256909/e64a623e6766/jvirol00159-0021-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3f1/256909/9b3f33255365/jvirol00159-0022-a.jpg

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引用本文的文献

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Microbiol Mol Biol Rev. 2003 Mar;67(1):86-156, table of contents. doi: 10.1128/MMBR.67.1.86-156.2003.
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Identification and biosynthesis of the bacteriophage T4 mot regulatory protein.噬菌体T4运动调节蛋白的鉴定与生物合成
EMBO J. 1983;2(7):1207-12. doi: 10.1002/j.1460-2075.1983.tb01568.x.
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本文引用的文献

1
Acetylornithinase of Escherichia coli: partial purification and some properties.大肠杆菌的乙酰鸟氨酸酶：部分纯化及某些性质
J Biol Chem. 1956 Jan;218(1):97-106.
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The amino acid composition of T3 bacteriophage.T3噬菌体的氨基酸组成。
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Phosphate-containing proteins of Salmonella typhimurium and Escherichia coli. Analysis by a new two-dimensional gel system.鼠伤寒沙门氏菌和大肠杆菌的含磷蛋白质。通过一种新的二维凝胶系统进行分析。
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Protein identifications of O'Farrell two-dimensional gels: locations of 81 Escherichia coli proteins.奥法雷尔双向凝胶的蛋白质鉴定：81种大肠杆菌蛋白质的位置
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Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
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The identification of prereplicative bacteriophage T4 proteins.复制前噬菌体T4蛋白的鉴定
J Biol Chem. 1973 Aug 10;248(15):5499-501.
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Ribonucleoside diphosphate reductase induced by bacteriophage Tr. I. Purification and characterization.
J Biol Chem. 1972 Nov 25;247(22):7270-5.
10
Membrane-associated proteins of T4-infected Escherichia coli.T4 感染的大肠杆菌的膜相关蛋白。
Virology. 1975 Aug;66(2):508-21. doi: 10.1016/0042-6822(75)90223-8.