二维凝胶蛋白上一些噬菌体T4复制前蛋白的鉴定
Identification of some bacteriophage T4 prereplicative proteins on two-dimensional gel proteins.
作者信息
Cook K S, Seasholtz A F
出版信息
J Virol. 1982 Jun;42(3):767-72. doi: 10.1128/JVI.42.3.767-772.1982.
Abstract
Profiles of bacteriophage T4 early proteins resolved by a two-dimensional nonequilibrium pH gradient electrophoresis system (P. Z. O'Farrell, H. M. Goodman, and P. H. O'Farrell, Cell 12:1133--1142, 1977) are presented. Over 65 phage-induced proteins were resolved. Amber or deletion mutants were used to identify 17 proteins in the gel patterns as the products of specific genes.
摘要
本文展示了通过二维非平衡pH梯度电泳系统解析的噬菌体T4早期蛋白质图谱（P.Z.奥法雷尔、H.M.古德曼和P.H.奥法雷尔，《细胞》12:1133 - 1142，1977年）。分辨出了超过65种噬菌体诱导的蛋白质。利用琥珀突变体或缺失突变体在凝胶图谱中鉴定出17种蛋白质为特定基因的产物。
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