Vihko P, Sajanti E, Jänne O, Peltonen L, Vihko R
Clin Chem. 1978 Nov;24(11):1915-9.
We describe radioimmunoassay for human prostatic acid phosphatase [orthophosphoric-monoester phospho-hydrolase (acid optimum), EC 3.1.3.2] in serum, with use of monospecific antisera raised in rabbits against highly purified acid phosphatase from human prostates. The antiserum did not cross react with partly purified acid phosphatases from human spleen, erythrocytes, or synovial tissues. 125I-labeled acid phosphatase was prepared by a Chloramine T method, and the bound and free antigen was separated in the assay by use of anti-rabbit gamma-globulin raised in sheep. Uniform low nonspecific binding of the [125I]acid phosphatase was achieved by using acid-phosphatase-free serum to prepare standard curves and diluted samples of serum with high acid phosphatase activities. Concentrations of immunoreactive acid phosphatase in the serum of healthy men ranged from less than 1 to 10 microgram/liter and for 12 patients with advanced prostatic carcinoma between 100 and 500 microgram/liter. The concentrations of the enzyme in sera of patients with benign prostatic hyperplasia were very similar to those in sera of the reference group.
我们描述了一种用于检测血清中人前列腺酸性磷酸酶[正磷酸单酯磷酸水解酶（最适酸性），EC 3.1.3.2]的放射免疫测定法，该方法使用了用兔制备的针对人前列腺高度纯化酸性磷酸酶的单特异性抗血清。该抗血清与人脾脏、红细胞或滑膜组织的部分纯化酸性磷酸酶无交叉反应。通过氯胺T法制备125I标记的酸性磷酸酶，并在测定中使用羊制备的抗兔γ球蛋白分离结合抗原和游离抗原。通过使用无酸性磷酸酶的血清制备标准曲线并用高酸性磷酸酶活性的血清稀释样品，实现了[125I]酸性磷酸酶均匀的低非特异性结合。健康男性血清中免疫反应性酸性磷酸酶的浓度范围为低于1至10微克/升，12例晚期前列腺癌患者的浓度在100至500微克/升之间。良性前列腺增生患者血清中该酶的浓度与参考组血清中的浓度非常相似。
