Proksch G J, Bonderman D P
Clin Chem. 1978 Nov;24(11):1924-6.
Cholesterol determination has been hampered by the lack of suitable means to prepare lyophilized serum control materials with an appropriate range of values. We prepared a water-soluble cholesterol derivative by first esterifying cholesterol with adipic acid and then reacting the cholesterol hemiadipate with Polyethylene Glycol 600 to form polyethoxyethanyl-cholesteryl adipate. The compound reacts quantitatively in several commonly used methods, including enzymic, extraction, and direct-assay procedures. When the additive is directly mixed with human serum that has been depleted of beta- and pre-beta-lipoproteins, an optically clear solution results for which cholesterol values are stable. The clarity is retained upon lyophilization and reconstitution. Addition of this cholesterol compound to partially delipidized serum appeared to have no significant effect on results of assay of 18 other commonly measured serum constituents.
由于缺乏合适的方法来制备具有适当值范围的冻干血清对照材料，胆固醇测定一直受到阻碍。我们通过首先将胆固醇与己二酸酯化，然后使胆固醇半己二酸酯与聚乙二醇600反应以形成聚乙氧基乙烷基胆固醇己二酸酯，制备了一种水溶性胆固醇衍生物。该化合物在几种常用方法中能进行定量反应，包括酶法、萃取法和直接测定法。当该添加剂直接与已去除β-和前β-脂蛋白的人血清混合时，会得到一种光学澄清的溶液，其胆固醇值稳定。冻干和复溶后仍能保持澄清。将这种胆固醇化合物添加到部分脱脂的血清中，似乎对其他18种常用测定的血清成分的测定结果没有显著影响。
