Kroll M H, Chesler R
Clinical Pathology Department, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892.
Clin Chem. 1990 Mar;36(3):534-7.
We determined the equilibrium absorbances and rate constants for two enzymatic methods, aca (DuPont) and RA-1000 (Technicon), used in determining cholesterol in reconstituted lyophilized serum. The lyophilized materials included two serum pools, three control materials, a College of American Pathologists' survey material, and Standard Reference Material no. 909. We calibrated the reagents with aca standards for cholesterol (DuPont). The difference in the mean concentrations of cholesterol (aca - RA-1000) was -0.09 g/L overall and was not statistically significant by analysis of variance. The mean rate constant for all materials was 0.23 min-1 for the aca and 1.42 min-1 for the RA-1000, significantly different (P less than 0.001). Lyophilization causes lower results for the aca method than for the Ra-1000, because the reaction rate for the aca method is slower and has not reached equilibrium when the final absorbance reading is made.
我们测定了用于测定冻干复溶血清中胆固醇的两种酶法（杜邦公司的aca法和泰康公司的RA - 1000法）的平衡吸光度和速率常数。冻干材料包括两个血清池、三种对照材料、一份美国病理学家协会的调查材料以及909号标准参考物质。我们用胆固醇的aca标准品（杜邦公司）对试剂进行了校准。胆固醇平均浓度（aca法 - RA - 1000法）的总体差异为 -0.09 g/L，经方差分析无统计学意义。所有材料的aca法平均速率常数为0.23 min⁻¹，RA - 1000法为1.42 min⁻¹，差异有统计学意义（P小于0.001）。冻干导致aca法的结果低于RA - 1000法，因为aca法的反应速率较慢，在进行最终吸光度读数时未达到平衡。
