Johnson J A, Lott H A
Clin Chem. 1978 Nov;24(11):1931-3.
Coomassie Brilliant Blue G-250 can be used to quantitatively determine proteins in cerebrospinal fluid. When the dye combines with protein, the absorption maximum of the dye shifts. The dye-protein color forms almost instantaneously and is stable for at least 1 h. The procedure is also insensitive to changes in temperature in the range of 20--30 degrees C. The absorptivities of the dye complexes with human albumin or globulin differ, thus a pure albumin or pure globulin standard is unsuitable; a standard containing 70% albumin and 30% globulins is the most appropriate for this application. A bichromatic approach to standardization increases the range of linearity of a calibration curve. The method gives values that are about 9% higher than a sodium sulfate-sulfosalicylic turbidimetric procedure for cerbrospinal fluid proteins.
考马斯亮蓝G - 250可用于定量测定脑脊液中的蛋白质。当该染料与蛋白质结合时，染料的最大吸收峰发生偏移。染料 - 蛋白质颜色几乎瞬间形成且至少稳定1小时。该方法在20 - 30摄氏度范围内对温度变化也不敏感。染料与人白蛋白或球蛋白形成的复合物的吸光率不同，因此纯白蛋白或纯球蛋白标准品不合适；含有70%白蛋白和30%球蛋白的标准品最适合此应用。采用双色标准化方法可增加校准曲线的线性范围。该方法得到的值比脑脊液蛋白质的硫酸钠 - 磺基水杨酸比浊法测得的值约高9%。
