对酸性磷酸酶动力学测定的干扰。
Interference with the kinetic determination of acid phosphatase.
作者信息
Sanders G T, Serné P, Hoek F J
出版信息
Clin Chim Acta. 1978 Nov 1;89(3):421-7. doi: 10.1016/0009-8981(78)90405-9.
This study advocates cautious use of the kinetic determination method for acid phosphatase with alpha-naphthylphosphate as substrate and a diazonium salt for colour development. The occurrence of considerable blank rates due to the spontaneous hydrolysis of the substrate was noticed. Of the diazonium salts which were tested as alternatives for Fast Red TR, diazotised p-nitroaniline seemed to be useful. Also the interaction between plasma components and diazonium salts may influence the results of the determination. In this respect a number of possible interfering compounds was investigated and from these a structural element which may lead to side reactions was deduced.
本研究提倡谨慎使用以α-萘基磷酸酯为底物、重氮盐显色的酸性磷酸酶动力学测定方法。注意到由于底物的自发水解会出现相当高的空白率。在作为固红TR替代品进行测试的重氮盐中，重氮化对硝基苯胺似乎是有用的。此外，血浆成分与重氮盐之间的相互作用可能会影响测定结果。在这方面，对一些可能的干扰化合物进行了研究，并从中推断出一种可能导致副反应的结构元素。
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