Ambler J
Clin Chim Acta. 1978 Nov 1;89(3):511-3. doi: 10.1016/0009-8981(78)90417-5.
A method is described for isoelectric focussing proteins on thick (500 micron) cellulose acetate gel membrane modified by boron trigluoride in methanol. Longer pH gradients could be formed and larger sample volumes could be applied than on the normal thickness product.